Production of Gericudranins by Hairy Root Culture of Cudrania tricuspidata

  • Seo, Weon-Taek (Bioprocess Technology Research Group Korea Research Institute of Bioscience and Biotechnology, Korea Institute of Science and Technology) ;
  • Lee, In-Kyoung (Bioprocess Technology Research Group Korea Research Institute of Bioscience and Biotechnology, Korea Institute of Science and Technology) ;
  • Yoo, Ick-Dong (Microbial Chemistry Research Group, Korea Research Institute of Bioscience and Biotechnology, Korea Institute of Science and Technology) ;
  • Park , Young-Hoon (Bioprocess Technology Research Group Korea Research Institute of Bioscience and Biotechnology, Korea Institute of Science and Technology)
  • Published : 1995.08.01

Abstract

Production of new flavanol derivatives with cytotoxic activity, gericudranin A and B, was studied by using hairy root cultures of Cudrania tricuspidata. Schenk and Hildebrandt (SH) medium was chosen for root growth and gericudranin production. After 35 days culture in a half-strength liquid SH medium containing $30g^{glucose}$/l, hairy root growth reached $138g^{FW}$/I and gericudranin A and B were produced at concentrations of 27mg/l and 21 mg/l, respectively. It was also observed that the contents of gericudranin A and B in hairy root were eight and six times higher than those of cudraniae radix, respectively.

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