Journal of Microbiology and Biotechnology
- Volume 5 Issue 4
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- Pages.200-205
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- 1995
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Nutritional Regulation of Morphological and Physiological Differentiation on Surface Culture of Streptomyces exfoliatus SMF13
- KYE JOON LEE (Department of Microbiology, College of Natural Science, and Research Center for Molecular Microbiology, Seoul National University) ;
- KIM, IN SEOP (Department of Microbiology, College of Natural Science, and Research Center for Molecular Microbiology, Seoul National University)
- Published : 1995.08.01
Abstract
Nutritional factors regulating the morphological differentiation and physiological differentiation of Streptomyces exfoliatus SMF13 on surface cultures were evaluated. S. exfoliatus SMF13 produced leupeptin and chymotrypsin-like protease (CTP) at the stage of substrate mycelium growth, and leupeptin-inactivating enzyme (LIE) and trypsin-like protease (TLP) at the stage of aerial mycelium growth. The activity of leupeptin and CTP was high in the region of active growing substrate mycelium, whereas the activity of LIE and TLP was high in the region of aerial mycelium or spores. The differentiations were induced in glucose-limited conditions or by the addition of glucose anti-metabolite (methyl
Keywords
- Streptomyces exfoliatus;
- trypsin-like protease;
- leupeptin;
- leupeptin-inactivating enzyme;
- morphological differentiation