Journal of Microbiology and Biotechnology
- Volume 5 Issue 2
- /
- Pages.110-113
- /
- 1995
- /
- 1017-7825(pISSN)
- /
- 1738-8872(eISSN)
Transposon Tn5 Mutagenesis of Bradyrhizobium japonicum: A Histidine Auxotrophic Mutant of B. japonicum Shows Defective Nodulation Phenotype on Soybean
Abstract
Transposon Tn5 was used to induce random insertional mutations in Bradyrhizobium japonicum, a soybean endosymbiont. By genomic Southern blot analysis, transposition events were found to have occurred randomly throughout the B. japonicum genome. After screening 3, 626 mutants by auxotrophy test, a histidine auxotroph was isolated. Upon plant infection test, the His mutant showed a 3~4 day delay in nodule formation.