Genetic analysis of polyketide biosynthetic genes isolated from Streptomyces albus, a salinomycin producer.

  • JOO-WON SUH (Biotechnology Research Institute, Myoung Ji University) ;
  • KWON, HYOUNG-JIN (Dept of Agricultural Chemistry and The Research Center for New Biomaterials in Agriculture Seoul National University) ;
  • C.R. HUTCHINSON (School of Pharmacy & Dept of Bacteriology, University of Wisconsin-Madison) ;
  • HYUNG-JONG JIN (School of Pharmacy & Dept of Bacteriology, University of Wisconsin-Madison) ;
  • SOO-UN KIM (Dept of Agricultural Chemistry and The Research Center for New Biomaterials in Agriculture Seoul National University) ;
  • KYE-JOON LEE (The Research Center for Molecular Microbiology, Seoul National University)
  • Published : 1995.04.01

Abstract

Sequence analysis of a DNA region encompassing the site of hybridization to actl, the gene for type II minimal polyketide synthase (PKS) for actinorhodin biosynthesis, from Streptomyces ablus revealed three more complete open reading frames additional to the already found two genes, plausibly encoding ${\beta}-ketoacyl$ synthase/acyl transferase (KS/AT) and chain length determining factor (ClF). The open reading frames (ORFs) were named salA, salD, and salE, from the upstream. In the homology analysis of the deduced amino acid sequences, SalA resembles the Streptomyces glaucescens Tcml, decaketide cyclase, SalD resembles acyl carrier protein in type II PKS, and SalE resembles the Actlll ketoreductase, The whole 4.4 kb of DNA sequence obeys the same conservation pattern as other type II PKSs. Therefore, we suggest that the 4.4 kb DNA from Streptomyces albus encompasses genes encoding enzymes for polyketide biogenesis in the organism and its organization is type II. The exsitence of SaIA, an analogue of the aromatic cyclase, revealed a relatedness of the 4.4 kb DNA with the aromatic PKS.

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