Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Regulation of Growth and Catharanthine Production by the Intracellular Phosphate Level in Hairy Root Cultures of Catharanthus roseus

세포내 인산농도에 의한 일일초 모상근생장 및 Catharanthine 생산의 조절

  • Kyung-Hee JUNG (Bioresources Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Sang-Soo KWAK (Bioresources Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Cha Yong Choi (Department of Chemical Technology, Seoul National University) ;
  • Jang R. LIU (Bioresources Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST)
  • Published : 1995.07.01
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Abstract

The relationship between the intracellular phosphate level and catharanthine production in hairy root cultures of Catharanthus roseus was investigated The growth of hairy root increased in proportion to the phosphate concentration in the growth medium. When hairy roots were cultured in a phosphate-free medium, the catharanthine content was increased to the highest level As the phosphate concentration in the medium was enhanced, the catharanthine content decreased proportionally. Hairy root cultures with elevated intracellular phosphate concentrations indicated that growth was proportional to the level of intracellular phosphate. Catharanthine production increased abruptly below an intracellular phosphate level of $100\;\mumoles/g$ dry wt. The intracellular phosphate level may play a key role in the regulation of growth and secondary metabolite production.

일일초 모상근배양에서 세포내 인산농도와 Catharanthine 생산과의 관계를 규명하였다. 모상근의 생장은 생장배지의 인산농도에 비례하여 증가하였다. Catharanthine 함량은 인산을 첨가하지 않은 배지에서 모상근을 배양하였을 때 최대값을 나타내었고 배지내의 인산농도를 증가함에 따라 비례적으로 감소하였다. 그리고 세포내의 인산농도에 비례하여 모상근의 생장은 증가하나 catharanthine의 함량은 감소하였다. Catharanthine 함량은 g 건조세포당 인산농도가 100mole 이하일 때에 현저하게 증가하였다. 이상의 결과로 세포내 인산농도는 모상근의 생장과 이차대사산물의 조절에 중요하게 관여함을 시사한다.

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