Streptomyces속 야생균주들이 생산하는 세포독성물질과 plasmid와의 연관성에 관한 연구

Studies on the Relationship Between the Presence of Plasmids and the Tumor cell's Cytotoxicity Shown by the Streptomyces spp

  • 김미용 (단국대학교 미생물학과) ;
  • 신석우 (단국대학교 미생물학과) ;
  • 최병돈 (단국대학교 미생물학과) ;
  • 염곤 (단국대학교 미생물학과)
  • Kim, Mi-Yong (Department of Microbiology, Dankook University) ;
  • Shin, Suck-Woo (Department of Microbiology, Dankook University) ;
  • Choi, Boung-Don (Department of Microbiology, Dankook University) ;
  • Ryeom, Kon (Department of Microbiology, Dankook University)
  • 발행 : 1995.06.01


We isolated Streptomyces spry. from Korean soil, which showed high cytotoxicity against tumor cell lines, L1210 and P388Dl. Among 30 strains, three strains (DKM 104, DKM 128, DKM 409) were appeared to possess plasmid. Strain DKM 104 and DKM 128 had two CCC(Covalently Closed Circular) form plasmid, about 20 Kb in size and about 1 Kb compared with λ Hind III DNA size marker. And strain DKM 409 had three plasmids, among which two plasmic were CCC form about 20 Kb and about 20 Kb compared with same size marker. To find out whether plasmid involved in production of antitumor agent or not, we performed to curing experiment. Comparing cytotoxicity between culture filtrate of plasmid-containing strains and cured strains, we knew that only the cytotoxic activity of the strain DKM 128 was involved in plasmid.