Environmental Mutagens and Carcinogens (한국환경성돌연변이발암원학회지)
- Volume 15 Issue 2
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- Pages.88-93
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- 1995
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- 1225-6307(pISSN)
Sensitization Effects of Hyperthermia on Bleomycininduced DNA Strand Breaks and Replication Inhibition in CHO-$K_1$ Cells in Vitro
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Kim, Chan-Gil
(Department of Molecular Biology, Konkuk University, Choongju) ;
- Kim, Hyeon-Suk (Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University) ;
- Park, Sang-Dai (Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University)
- Published : 1995.09.01
Abstract
Effects of hyperthermia on the induction of DNA single strand breaks and replication inhibition were studied in bleomycin-treated CHO-K$_1$ cells by alkaline elution and alkaline sucrose gradient sedimentation. Bleomycin-induced DNA single strand breaks of DNA were dose-and time-dependently increased, and these strand breaks of DNA were gradually rejoined as post-incubation time passed. Treatment with hyperthermia alone did not affect the induction of DNA single strand breaks. However, pre-exposure of cells to hyperthermia followed by bleomycin treatment greatly increased the single strand breaks, and also reduced the rejoining processes of bleomycin-induced DNA single strand breaks. Bleomycin selectively inhibited the replicon initiation. The combined treatment with hyperthermia and bleomycin markedly potentiated the nonspecific inhibition of replication.
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