연두금파리의 난세포성숙에 따른 단백질의 변화와 난특이성단백질의 특성

Protein Contents During Oocyte Development and Some Characteristics of Egg-Specific Protein in Lucilia illustris

  • Lee, Jong-Jin (Department of Agricultural Biology, Chonbuk National University) ;
  • Man-Young Choi (Department of Agricultural Biology, Chonbuk National University) ;
  • Hee-Kwon Lee (Department of Agricultural Biology, Chonbuk National University)
  • 발행 : 1995.06.01

초록

연두금파리의 난세포성숙에 따른 단백질의 변화와 난특이성단백질의 특성을 확인하기 위하여 gel filtration, 전기영동 및 분자량측정, 아미노산과 지방산함량을 측정하여 얻은 결과는 다음과같다. 연두금파리 암컷성충의 난소단백질은 단백질원을 섭식시킨 후 72시간 이후 빠르게 증가하였고, 완전한 성숙이 일어나는 96시간에 최고의 함량을 나타냈다. DEAE-cellulose와 Sephacryl 5-200으로 gel filtration하고 7.5% native polyacrylamide gel에서 전기영동한 결과 난소에서 분리된 특이단백질은 ${R}_{f}$ 0.4에서 혈림프 및 난소와 다른 밴드가 확인되었으며, 분자량은 110,000 dalton이였다. 분리된 난특이성단백질내 아미노산 조성은 asparagine 외 모두 13종이 검출되었으며, asparagine, glutamic acid와 함께 tyrosine이 특이하게 높게 나타났다. 지방산은 난소와 함께 난특이성단백질에서 palmitic acid의 4종이 분리되었다. 따라서, 연두금파리의 난에는 지방체에서 합성, 분비된 난황단백질이외에 난소에만 존재하는 특이단백질이 있음을 알 수 있다.

Changes in protein content during oocyte development was measured and egg-specific protein was characterized from the eggs in Lucilia illustris. During normal development ovarian protein was rapidly increased at 72hr and reached maximum at 96hr after a protein meal, when the eggs were fully matured. Purified protein from the ovaries by gel filtration of DEAE-cellulose an Sephacryl S-200 was loaded on 7.5% native polyacrylamide gel electrophoresis and identified at ${R}_{f}$ 0.4 as egg-specific protein, which has a mol. wt of 110,000. A total of 13 amino acids in th egg-specific protein was identified and expecially asparagine, glutamic acid, and tyrosine were highly concentrated. Five fatty acids were also identified. It is suggested that there is a specific protein in the eggs of L. illustris except yolk protein synthesized and secreted by fat body.

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