Journal of Nutrition and Health

  • 제28권2호
  • /
  • Pages.115-126
  • /
  • 1995
  • /
  • 2288-3886(pISSN)
  • /
  • 2288-3959(eISSN)
한국영양학회 (The Korean Nutrition Society)
권호 표지

발현성 고혈압쥐의 관상순환 기능과 심장근의 에너지 대사에 관한 생체외 에너지원의 관류 연구

Substrate-Perfusion Studies on Coronary Circulation and Myocardial Energy Metabolism in Spontaneously Hypertensive Rat Hearts

  • 김은지 (한림대학교 자연과학대학 식품영양학과)
  • 발행 : 1995.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The effects of energy-yielding substrates on coronary circulation, myocardial oxygen metabolism, and intramyocytic adenylates of perfused Wistar control rat(WC) and spontaneously hypertensive rat(SHR) hearts were examined under basal and $\beta$-adrenergic stimulation conditions. The perfusion medium (1.0mM Ca2+) contained 5mM glucose (+5U/l insulin) in combination with 5mM pyruvate, 5mM lacate, 5mM acetate, or 5mM octanoate as energy substrates. Hearts were perfused with each substrate buffer for 20min under basal conditions. Coronary functinal hyperemia was induced by infusing for 20min isoproterenol (ISO, 1uM), a $\beta$-receptor agonist. Cardiac adenylates, glycolytic intermediates, and coronary venous lactate were measured by using an enzymatic analysis technique. Under basal conditions, acetate and octanoate significantly increased coronary flow(CF) of WC in parallel with myocardial oxygen consumption. However, CF of SHR was partly attenuated by coronary vasoconstriction despite metabolic acidosis. In addition, pyruvate and lactate depressd ISO-induced coronary functional hyperemia in SHR. It should be noted that octanoate exhibited coronary dysfunction under ISO conditions. On the other hand, fat substrates depleted myocardial high energy phosphate pool and accumulated breakdown intermediates. In SHR with coronary vasoconstriction under basal conditions, and with depressed coronary functional hyperemia, high energy phosphates were greatly depleted. These results suggest that energy substrates in the myocardium and coronary smooth muscle alter remarkably coronary circulation, and that coronary circulatory function is associated with a reserve of high energy phosphates and a balance between breakdown and nono synthesis of energy phosphates. These findings could be explained by alterations in the cytosolic redox state manipulated by LDH and hence in the cytosolic phosphorylation potential, which might be involved in hypertension of SHR.

키워드

참고문헌

  1. Circulation v.87 Hypertension and left ventricular hypertrophy are associated with impaired endothelium-mediated in human coronary resistance vessels Treasure,C.B.;Klein,J.L.;Vita,J.A.;Manoukian,S.V.;Renwick,G.H.;Selwyn,A.P.;Ganz,P.;Alexander,R.W.
  2. Hypertension v.22 Possible genetic influence on the strength of human muscle sympathetic activity at rest Wallin,B.G.;Kunimoto,M.M.;Sellgren,J.
  3. Am J Physiol v.264 Mechanisms for altered endothelium-dependent vasorelaxation in isolated kidneys from experimental hypertensive rats Hayakawa,H.;Hirata,Y.;Suzuksi,E.;Sugimoto,T.;Matsuoka,H.;Kikuchi,K.;Nagano,T.;Hirobe,M.;Sugimoto,T.
  4. Am J Physiol v.264 Enhanced single-channel K+ current in arterial membranes from genetically hypertensive rats England,S.K.;Woodridge,T.A.
  5. Hypertension v.8 Alterations in plasma membrane properties of the myocardium of spontaneously hypertensive rats Sharma,R.V.;Butters,C.A.;Bhalla,R.C.
  6. Hypertension v.22 Rested-state contractions and rest potentiation in spontaneously hypertensive rats Perez,G.N.;Petroff,M.V.;Mattiazzi,A.
  7. Life Sci v.37 Vasopressin-induced calcium increases in smooth muscle cells from spontaneously hypertensive rats Nabika,T.;Velletri,P.A.;Beaven,M.A.;Endo,J.;Lovenberg,W.
  8. J Hypertension v.10 Enhanced spontaneous calcium efflux and decrease of calcium-dependent calcium release from isolated perfused heart of spontaneously hypertensive rats Hojo,Y.;Ebata,H.;Ikeda,U.;Tsuruya,T.;Natsume,T.;Shimada,K.
  9. Am J Physiol v.199 Pressure-flow relationship in the coronary vascular bed of the dog Scott,J.B.;Hardin,R.A.;Haddy,F.J.
  10. Circ Res v.13 Effect of coronary perfusion pressure or coronary flow on oxygen usage of the myocardium Gregg,D.E.
  11. Am J Physiol v.243 Role of mitochodrial oxidative phosphorylation in regulation of coronary blood flow Nuutinen,E.M.;Nishiki,K.;Erecinska,M.;Wilson,D.F.
  12. Pfl$\"{u}$gers Arch v.421 Coronary autoregulation and purine release in normoxic heart at various cytoplasmic phosphorylation potentials : Disparate effects of adenosine Kang,Y.H.;Mallet,R.T.;Bunger,R.
  13. Role of adenosine and adenine nucleotides in the biological system Guinea pig cardiac free, bound and interstitial adenylates : Energy linked and energy-independent adenosine release Bunger R;Mallet RT;Kang YH;Imai S(ed.);Nakazawa M(ed.)
  14. Am J Physiol v.250 Supply-to-demand ratio for oxygen determines formation of adenosine by the heart Badenheuer,H.;Schrader,J.
  15. Am J Physiol v.264 Adenosine regulates coronary flow during increased work and decreased supply Martin,S.E.;Lenhard,S.D.;Schmarkey,L.S.;Offenbacher,S.;Odle,B.M.
  16. Pfl$\"{u}$ugers Arch v.353 An isolated guinea pig heart preparation in vivo-like features Bunger,R.;Haddy,F.J.;Querengasser,A.;Gerlach,E.
  17. Circ Res v.42 Adenosine, theophylline and perfusate pH in the isolated, perfused guinea pig heart Merrill,G.F.;Haddy,F.J.;Dabney,J.M.
  18. 한국영양학회지 v.25 no.7 Guinea pig heart의 관상 동맥 순환기능과 calcium release에 있어서 caffeine이 미치는 영향 김은지;김은미;강영희
  19. Eur J Biochem v.159 Cytosolic adenylates and adenosine release in perfused working heart : Comparison of whole tissue with cytosolic non-aqueous fractionation analyses Bunger,R.;Soboll,S.
  20. Circ Res v.23 Early changes in energy metabolism in the myocardium following acute coronary artery occlusion in anesthetized dogs Braasch,W.;Gudbjarnason,S.;Purl,P.S.;Ravens,K.G.;Bing,R.J.
  21. Eur J Biochem v.188 Glucose requirement for postischemic recovery of perfused working heart Mallet,R.T.;Hartman,D.A.;Bunger,R.
  22. Prog Cardiovasc Dis v.24 Metabolic control of coronary blood flow Olsson,R.A.;Bunger,R.
  23. Am J Physiol v.264 Pyruvate and lactate metabolism in the in vivo dog heart Laughlin,M.R.;Taylor,J.;Chesnick,A.S.;DeGroot,M.;Balaban,R.S.
  24. Jpn Heart J v.15 ATPase activity and $Ca^ {2+}$ interaction of myofibrils and sarcoplasmic reticulum isolated from the hearts of spontaneously hypertensive rats Aoki,K.;Ikeda,N.;Yamashita,K.;Hotta,K.
  25. Proc Natl Acad Sci v.83 Decreased content of integral membrane calcium-binding protein(IMCAL) in tissues of spontaneously hypertensive rat Kowarski,S.;Cowen,I.;Schachter,D.
  26. 순환기 v.24 no.5 Actions of isoproterenol for coronary circulation in spontaneously hypertensive rat hearts 김은지;강영희
  27. Circulation v.88 no.Supp Altered relaxation and Sarcoplasmic Reticulum calcium content in cardiac muscle from the aging spontaneously hypertensive rat Moravec,C.S.;Kelly,E.;Bond,M.
  28. Hypertension v.3 Impaired cardiac contractile response to isoproterenol in the spontaneously hypertensive rat Saragoca,M.;Tarazi,R.
  29. Am J Physiol v.252 Vanhoutte-dependent contraction to stretch in canine basilar arteries Katusic,F.S.;Shepherd,J.T.;Vanhoutte,P.M.
  30. N Engl J Med v.315 Paradoxical vasoconstriction produced by acethylcholine in atherosclerotic coronary arteries Ludmer,P.L.;Selwyn,A.P.;Shook,R.L.
  31. Hypertension v.8 no.SuppⅡ The loss of endothelium-dependent vascular relaxation in hypertension Lokette,W.;Otsuka,Y.;Carretero,O.
  32. Hypertension v.8 Endothelium-dependent contraction to acetylcholine in the aorta of the spontaneously hypertensive rat Luscher,T.F.;Vanhoutte,P.M.