Abstract
These studies were carried out to investigate the effects of Puerariae radix catechins(PRC) administration on the biochemical parameters of liver function in liver of carbon tetrachloride(CCl4)-treated rats. Thirty six healthy Sprague-Dawley rats weighing about 120g were used for this experiment and divided intot he following 3 groups : normal control group(NCON), $CCl_4$ control group(CCON), PRC treated group(PRC). Fifty percent $CCl_4$ in oil was administered(I.P.) by 2ml per kg body weight two times a week for 3 weeks. PRC treated groups were administered orally at the leaves of 1% per day in distilled water for 8 weeks. Lipid hydroperoxides were analyzed by using chemiluminescence-high performance liquid chromatography(CL-HPLC) method as a phosphatidylcholine hydroperoxide value(PCOOH) in liver tissues. $CCl_4$ treatment significantly(p<0.05) resulted in an increase in GPT & GOT activities and liver hydroperoxide values comparing with those of the untreated control, while administration of PRC to the $CCl_4-treated$ rats significantly(p<0.001) decreased GPT & GOT activities and liver hydroperoxide value. Their ultrastructual changes of hepatocellular organelles were shown to clarify the morphologic nature of protective effects of PRC on hepatocytic injuries. $CCl_4$ treatment observed to change the ultrastructual nature of outer membrane of hepatocytes. However, the hepatic changes on PRC treatment to $CCl_4$ group was not found. PRC administration may inhibit the formatiion of liver lipid hydroperoxides in vivo and were very effective in recovering the liver function in $CCl_4-treated$ rats.
본 연구는 3주간 $CCl_4$를 투여한 랫트를 이용하여 각 장기 및 혈액 생화학치의 변화를 조사, 과산화지질가를 감도가 좋은 형광발광 액체 크로마토그래피를 이용해 측정, 간세포를 전자현미경적으로 관찰 등의 방밥으로 간기능에 미치는 PRC의 효과를 알아보기 위하여 실시하였다. 그 결과, PRC투여군은 정상대조군에 비하여 체중은 감소하고, 상대적 간장 중량의 감소경향, GOT 및 GPT의 수치가 감소하였으며, 특히 과산화물가가 유의하게(p<0.001) 감소하였다. 이로 인한 결과를 명확히 뒷받침해 주고 있는 간세포에 대한 전자현미경적 관찰의 결과는 RPC투여의 효과를 간세포의 형태학적 관찰에서 간세포에 대한 PRC의 효과를 나타내 주었다. 이 결과는 칡 카테킨이 사염화탄소로 유도된 간장의 과산화지질의 형성을 방지해서 간기능을 회복시키는데 효과적이라는 것을 시사한다 하겠다.
