한국식품영양학회지 (The Korean Journal of Food And Nutrition)
- 제8권1호
- /
- Pages.43-49
- /
- 1995
- /
- 1225-4339(pISSN)
- /
- 2287-4992(eISSN)
Chitosanase의 분해에 의한 Chitooligo당의 분리 정제
Purification and Seperation of Chitooligosaccharides Hydrolyzed by Chitosanolytic Enzyme
- Ryu, Byeong-Ho (Dept. of Food Eng., Kyung-Sung University) ;
- Bin, Jae-Hun ;
- Lee, Seong-Ho
- 발행 : 1995.03.01
초록
This studies were carried out to purification and seperation of chitooligosaccharides which containing excellent biological active substance. After deacetylation of chitosan (DAC%), DAC-45%, DAC-70%, DAC-95% and DAC-99% were used substrates and hydrolyzed by chitosanase (Bacillus pumilus BN-262) DAC-99% has excellent hydrolyzate which contained several chitooligosaccharides. Therefore, chitosan was hydrolyzed DAC-90 as substrate by chitosanase, and then purified and seperated of chitooligosaccharides Gel filteration and HPLC. This oligosaccharides composed with GlcN0, GlcN2, GlcN3, Glc5 and GlcN6.