The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Purification and Seperation of Chitooligosaccharides Hydrolyzed by Chitosanolytic Enzyme

Chitosanase의 분해에 의한 Chitooligo당의 분리 정제

  • 류병호 (경성대학교 식품공학과) ;
  • 빈재훈 (경성대학교 식품공학과, 경남전문대학 식품가공과) ;
  • 이성호
  • Published : 1995.03.01
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Abstract

This studies were carried out to purification and seperation of chitooligosaccharides which containing excellent biological active substance. After deacetylation of chitosan (DAC%), DAC-45%, DAC-70%, DAC-95% and DAC-99% were used substrates and hydrolyzed by chitosanase (Bacillus pumilus BN-262) DAC-99% has excellent hydrolyzate which contained several chitooligosaccharides. Therefore, chitosan was hydrolyzed DAC-90 as substrate by chitosanase, and then purified and seperated of chitooligosaccharides Gel filteration and HPLC. This oligosaccharides composed with GlcN0, GlcN2, GlcN3, Glc5 and GlcN6.

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