Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 10 Issue 2
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- Pages.105-113
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- 1995
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Production of Nuclear Transplant Embryos Using Follicular Oocytes in Rabbits
토끼에서 난포란을 이용한 핵이식배 생산에 관한 연구
Abstract
This study was conducted to examine the efficiency of enucleation and blastomere isolation from recipient oocytes and donor embryos, respectively and to determine the effect of oocyte age and electric voltage on the fusion rate and in vitro development of the fused oocytes in rabbit nuclear transplantation. Immature oocytes collected from ovarian follicles were matured in vivo for 12 h in TCM-199 containing FCS and hormones and in vivo matured oocytes were collected 17 to 18 h post-HCG. The fresh and frozen donor embryos of 8- to 16-cell stage were collected from the oviduct of superovulated does. The proportion of successfully enucleated oocytes was greatly lower in in vitro matured oocytes (42.3%) than that (62.7%) in in vivo matured oocytes The level of cytochalasin B for in vivo matured oocytes did not affect the efficiency of enuleation, but 7.5