Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Microorganism Producing Chitinase for Chitooligosaccharides Production, Purification of Chitinase, and its Enzymatic Characteristics

Chitoologosaccharides 생산에 적합한 Chitinase를 분비하는 균주의 선별, Chitinase의 분리정제 및 반응특성

  • 정의준 (경북대학교 자연과학대학 유전공학과) ;
  • 이용현 (경북대학교 자연과학대학 유전공학과)
  • Published : 1995.05.01
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Abstract

In order to produce fuctional chitooligosaccharides, a strain excreting mainly endo-type chitinase suitable for chitooligosaccharides production was newly screened and identified as Aspergillus fumigatus JC-19. The chitinase excretion was repressed in nutrient rich medium but stimulated by colloidal chitin indicating that the chitinase is inducible type enzyme. Maximum secretion of the enzyme was observed at pH 7.0 and 37$\circ$C . The growth and chitinase production patterns of Aspergillus fumigatus JC-19 showed that the cell growth reached maximum after 4-5 days with final chitinase concentration of 0.46 unit per ml. Excreted chitinase was purified by ammonium sulfate precipitation, colloidal chitin adsorption, anion exchange chromatography, and gel filtration, respectively, and measured M.W of 50 KDa. The enzyme reaction carried out both by crude and purified chitinase showed that the purified chitinase accumulated more chitooligosaccharides of 1-6 degree of polymerization than that of crude chitinase.

Keywords

References

  1. 주류 공업 v.43 Chitin/Chitosan 유도체의 구조, 분해효소, 이용 및 주류 산업에의 활용 이용현;정의준
  2. 最後の Biomass. Chitin and Chitosan 日本 フツドケミカル
  3. Chitin Muzzarelli,R.A.A.
  4. Biochim. Biophys. Acta. v.923 Transglycosylation reaction of a chitinase purified from Norcardia orientalis Usui,T.;Y.Hayashi;F.Nanjo;K.Sakai;Y.Ishido
  5. Biochim. Biophys. Acta. v.1078 Isolation and characterization of thermostable chitinase from Bacillus licheniformis X-7U Takayanagi,T.;K.Ajisaka;Y.Takiguchi;K.Shimahara
  6. Arch. Int. Physiol. Biochem. v.67 Recherches sur les chitinases.2. Purification de la chitinase d'un Streptomycte et separation electrophoretique de principes chitinolytiques distincts Jeuniaux,C.
  7. Experientia v.48 What's new in chitinase research? Flach,J.;P.E.Pilet;P.Jolles
  8. World J. Microbiol. Biotechnol. v.9 Chitinolytic enzymes: their contribution to basic and applied research Shaikh,S.A.;M.V.Deshpande
  9. J. Gen. Microbiol. v.135 Purification and some properties of two forms of chitinase from mycelial cells of Mucor rouxii Pedraza-Reyes,M.;E.Lopez-Romero
  10. Meth. Enzymol. v.161 Biomass Pt B. lignin. pctin and chitin Wood,W.A.;S.T.Kellogg
  11. Plant Physiol. v.87 Antifungal hydrolases in pea tissue.Ⅰ. Purification and characterization of two chitinases and two β-1.3-glucanases differentially regulated during development and in response Mauch,F.;L.A.Hadwinger;T.Boller
  12. Food Chemical, Chitin/Chitosan の 科學 平野茂博;憧口泰之;島原健三
  13. Meth. Enzymol. v.8 Chitinases Jeuniaux,C.
  14. Introduction to Food-Borne Fungi(3rd ed.) Samson,R.A.;E.Van Reenen-Hoekstra
  15. Compendium of Soil Fungi Domsch,K.H.;W.Gams;T.H.Anderson
  16. The Genus Aspergillus Raper,K.E.;D.I.Fennell
  17. Protein Methods Daniel,M.B.;J.E.Stuart
  18. J. Gen. Appl. Microbiol. v.32 Purification and characterization of chitinase and chitobiase produced by Aeromonas hydrophila ssp. Anaerogenes A52 Yabuki,M.;K.Mizushina;T.Amatatsu;A.Ando;T.Fujii;M.Shimada;M.Yamashita
  19. J. Bacteriol. v.56 The utilization of carbon compounds by some Actinomycetales as aids for species determination Pridham,T.G.;D.Gottlieb
  20. J. Gen. Microbiol. v.11 Exocellular chitinase from a Streptomyces sp. Reynolds,D.M.
  21. Biochim. Biophys. Acta. v.29 Chitinase system of a strain of Streptomyces griseus Berger,L.R.;D.M.Reynolds
  22. Enzymologia v.39 Chitinase of Streptomyces sp. purification and properties Skujins,J.;A.Pukite;A.D.Melaren