Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Screening of Antifungal Compounds from Microorganisms with Preferential Activity against the Mycelial Phase of Candida albicans

토양으로부터 Candida albicans 의 균사형태에 선택적인 활성을 나타내는 미생물로부터 항진균 물질의 탐색

  • 김성욱 (한국과학기술연구원 유전공학연구소 생물소재연구그룹) ;
  • 남지연 (한국과학기술연구원 유전공학연구소 생물소재연구그룹) ;
  • 권병목 (한국과학기술연구원 유전공학연구소 생물소재연구그룹) ;
  • 손광희 (한국과학기술연구원 유전공학연구소 생물소재연구그룹) ;
  • 복성해 (한국과학기술연구원 유전공학연구소 생물소재연구그룹)
  • Published : 1995.05.01
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Abstract

To search and develop the compounds exhibiting antifungal activities against the mycelial phase of Candida albicans, approximately 2,900 microorganisms isolated from soil were examined for antifungal activity. Among them, a strain with preferential activity against the mycelial phase of Candida albicans was isolated and identified as Streptomyces sp. A393. Isolation and purification of compounds A393 showing antifungal activity against the mycelial phase of C. albicans were performed using XAD-7 column chromatography, silica gel chromatography, preparative thin- layer silica gel chromatography, and HPLC. The molecular weights of compounds isolated from Streptomyces sp. A393 were determined as 774, 790, 804 and 820. These compounds appeared to have a structure of macrolide antibiotics, oligomycin A, B, C and E. Especially, oligomycin E, which is formerly reported to have no antifungal activity, showed antifungal activity against the mycelial phase of Candida albicans.

Keywords

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