한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제23권2호
  • /
  • Pages.145-149
  • /
  • 1995
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Xanthomonas sp. YL-37의 Alkaline Protease 유전자의 클로닝

Cloning of a Alkaline Protease Gene from Xanthomonas sp. YL-37

  • 이대희 (경남대학교 식품공학과) ;
  • 김수경 (경남대학교 식품공학과) ;
  • 이승철 ;
  • 윤병대 (경남대학교 식품공학과) ;
  • 황용일
  • 발행 : 1995.05.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

For the purpose of developing a new biodegradable detergent, we have isolated a gene encoding wide-range temperature applicable alkaline protease from Xanthomonas sp. YL-37 (Lee et al., 1994, Kor. J. Appl. Microbiol. Biotechnol.). An alkaline protease gene was isolated from the gene bank that was prepared from the chromosomal DNA of Xanthomonas sp. YL-37. From the results of agarose gel electrophoresis and a restriction enzyme mapping, a 2.7 kb DNA fragment containing the alkaline protease gene was inserted in the plasmid pUC9. Extracellular activity of a clone having alkaline protease gene was detected on SDS-polyacrylamide gel with activity staining assay. The molecular weight of alkaline protease was determined to be about 64 kDa from 11% SDS-PAGE analysis. Alkaline protease activity, produced from E. coli which harboring the plasmid, showed no difference at reaction temperature 20, 30 and 40$\circ$C, respectively. This result showed that alkaline protease produced from E. coli harboring the plasmid was apparently the same as that of Xanthomonas sp. YL-37.

키워드

참고문헌

  1. Biotechnol. Bioeng. v.5 Pure culture studies of the degradation of detergent compounds Paye,W.J.
  2. Surfactant biodegradation Swisher,R.D.
  3. Cationic surfactant Jungermann,E.
  4. Agric. Biol. Chem. v.48 Purification and properties of acid protease from Monascus sp. No. 3405 Masaaki,Y.;S.Kazuo;M.Mitsuo
  5. Agric. Biol. Chem. v.35 Production of alkaline enzymes by alkalophilic microorganism Horikoshi,K.
  6. 한국농화학회지 v.31 Bacillus subtilis가 생산하는 alkaline protease에 관한 연구 장신재;김윤숙;성하진;최용준;양한철
  7. 한국산업미생물학회지 v.18 호알카리성 Bacillus 속 균주가 생산하는 alkaline protease의 특성 김태호;박성희;이동선;권택규;김종국;홍순덕
  8. Biochem. Biophys. Acta. v.869 Specificities of extracellular and ribosomal serine proteinases from Bacillus natto. a food microorganism Ichishima,E.;V.Takada;K.Taira;M.Takduchi
  9. 한국산업미생물학회지 v.22 Bacillus sp. SH-8과 Bacillus sp. SH-8M의 Protease 생산 및 특성에 미치는 영향 심창환;정광선;신원철;유주현
  10. Agric. Biol. Chem. v.38 Purification and some properties of an alkalophilic proteinase of a Streptomyces species Nakanish,T.;Y.Matamura;N.Minamiura;T.Yamamoto
  11. 한국산업미생물학회지 v.17 Streptomyces rimous가 생성하는 protease의 정제와 특성 김경미;이태경;양한철
  12. 한국산업미생물학회지 v.17 Streptomyces sp. YSA-130이 생산하는 alkaline protease의 정제와 특성 윤성우;이강표;윤주현;신철수;오두환
  13. J. Biol. Chem. v.247 The proteolytic enzymes of the K-1 strain of Streptomyces griseus obtained from commercial preparation Siegel,S.;A.H.Brady;W.M.Awad
  14. 한국농화학회지 v.27 Streptomyces alboniger가 생산하는 protease의 특성에 관한 연구 김경신;한강완;김형로
  15. Appl. Env. Microbiol. v.57 Production of alkaline protease by a new Aspergillus flavus isolate under solid-substrate fermentation conditions for use as a depilation agent Malathi,S.;R.Chakraborty
  16. 한국영양식량학회지 v.18 Aspergillus fumigatus에 의한 alkaline protease의 생산과 정제 차원섭;조영제;최청
  17. Yakugaku Zasshi v.88 Studies on Aspergillus proteinase Ito,M.;M.Sugiura
  18. 한국산업미생물학회지 v.22 알카리성 protease를 생산하는 Xanthomonas sp. YL-37의 분리 및 조효소의 성질 이창호;권태종;강상모;서현호;권기석;오희목;윤병대
  19. J. Bacteriol. v.169 Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis Antoine,A.;F.Kunst;E.Aubert;A.Klier;G.Rapoport
  20. Molecular cloning(2nd ed.) Sambrook,J.;E.F.Fritsch;T.Maniatis
  21. Cell v.18 Isolation of yeast histidine genes H2A and H2B Hereford,L.;K.Fahrner;J.Woolford;M.Rosbash;D.B.Kaback
  22. Proc. Natl. Acad. Sci. USA v.62 Supercoiled circular DNA protein complex in Escherichia coli: Purification and induced conversion to an open circular form Clewell,D.S.;Helinski,D.R.
  23. J. Bacteriol. v.132 Transfomation in Escherichia coli: cryogenic preservation of competent cells Morrison,D.A.
  24. Nature v.227 Clevage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli,U.K.
  25. Anal. Biochem. v.102 Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates Christa,H.;E.B.Dowdle
  26. 한국산업미생물학회지 v.20 Streptomyces 속 균주가 생성하는 Alkaline Protease의 생산 및 정제 최청;정영건;성삼경;최광수;이재성;조영제;권오진
  27. J. Bacteriol. v.169 Characterization of Erwinia chrysanthemi extracellular proteases: cloning and expression of the protease genes in Escherichia coli Cecile,W.;P.Delepelaire;S.Letoffe;M.Schwartz