Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Chemical Synthesis of a Human Lysozyme Gene and Expression in Saccharomyces cerervisiae

Human Lysozyme 유전자의 화학적 합성과 Saccharomyces cerevisiae 에서의 발현

  • 김기운 (경남대학교 공과대학 식품공학과) ;
  • 이승철 (경남대학교 공과대학 식품공학과) ;
  • 황용일 (경남대학교 공과대학 식품공학과)
  • Published : 1995.05.01
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Abstract

The cDNA, encoding human lysozyme (HLY) which was isolated from a human placenta cDNA library, has been well characterized (Yoshimura et al., 1988). Based on the communication, we have prepared an artificial HLY gene from chemically synthesized 38-oligomer with high codon usage in Saccharomyces cerevisiae. For directing the synthesis and secretion of HLY in S. cerevisiae, an expression vector, pHKl was constructed by inserting the HLY gene, containing a synthetic HLY secretion signal sequence, between the yeast GAP promoter and PH05 terminator. From a lysoplate assay, we have confirmed an yeast transformant harboring a pHK1 which makes a clearing zone on the overlayed Micrococcus luteus. This result means a chemically synthesized HLY gene which was normally expressed and secreted in yeast.

Keywords

References

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