Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Espression of Alkaline Phosphatase Gene from Kluyveromyces fragilis in E. coli and S. cerevisiae

Kluyveromyces fragilis의 Alkaline Phosphatase 유전자의 E. coli 및 S. cerevisiae 에서의 발현

  • 박수영 (경북대학교 자연과학대학 미생물학과) ;
  • 황선갑 (경북대학교 자연과학대학 미생물학과) ;
  • 이동선 (경북대학교 자연과학대학 미생물학과) ;
  • 김종국 (경북대학교 자연과학대학 미생물학과) ;
  • 남주현 (대구공업전문대학 식품공업과) ;
  • 홍순덕 (경북대학교 자연과학대학 미생물학과)
  • Published : 1995.05.01
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Abstract

The alkaline phosphatase (K-ALPase) gene of Kluyveromyces fragilis has been cloned (1) and determined its base sequences (2) previously in our laboratory. When the K-ALPase gene was expressed in Escherichia coli and Saccharomyces cerevisiae, it showed a constitutive activity in E. coli, and a derepressed activity in S. cerevisiae in phosphate-limited medium. Northem hybridization experiment was performed to elucidate the transcription level of the K-ALPase gene. Northern experiment showed that transcription level of K-ALPase gene in S. cerevisiae was higher in phosphate depletion, but it was higher in high phosphate medium than in phosphate limited medium in K. fragilis. The transcription initiation site of the K-ALPase gene was determined by primer extension analysis. It matched nucleotide position - 169 in relation to the putative trnslational start site.

Keywords

References

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