Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immunological Characterization of Bacillus thuringiensis Antigens

Bacillus thuringiensis 항원들의 면역학적 분석

  • Jung, Jae-Deuk ;
  • Park, Jung-Sun ;
  • Jo, Young-Soo ;
  • Hong, Soon-Bok ;
  • Lee, Hyung-Hoan ;
  • Cho, Myung-Hwan
  • 정재득 (건국대학교 생물학과) ;
  • 박정선 (건국대학교 생물학과) ;
  • 조영수 (건국대학교 생물학과) ;
  • 홍순복 (건국대학교 생물학과) ;
  • 이형환 (건국대학교 생물학과) ;
  • 조명환 (건국대학교 생물학과)
  • Published : 1995.02.01
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Abstract

This study was carried out to immunologically characterize Bacillus thuringiensis (B.t) antigens. Protein patterns of ultrasonicated- antigens of B. thuringiensis subspecies using SDS- PAGE revealed marked similarities among all the strains analyzed except for the difference between quantative variations of bands and some protein antigens. The comparison of the protein patterns showed that the protein antigen of 45 kilodalton (kd) was common in 11 strains and that the difference between B. thuringiensis subsp. canadensis and galleriae was noticed in quantative variations of bands despite of ambiguous serogrouping, suggesting a useful method for identification. All strains examined showed similar antigenic patterns in SDS-PAGE, while immunodominant bands differed in antigenic reactivity in western blot using polyclonal antibodies. Polyclonal antibody to B. thuringiensis subsp. thuringiensis and israelensis in indirect immunofluorescence assay reacted with flagella and cell surface antigens. The present study indicates that SDS-PAGE and western blot analysis may be used as tools for differentiation and identification of B. thuringiensis subspecies.

Keywords

References

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