Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization and Structural Dtercination of an Antifungal Compound Produced by Pseudomonas aeruginosa KGM-100

Pseudomonas aeruginosa KGM-100이 생산하는 항생물질의 특성 및 구조

  • 김경석 (경북대학교 자연과학대학 유전공학과) ;
  • 홍수형 (경북대학교 자연과학대학 유전공학과) ;
  • 이은주 (경북대학교 자연과학대학 유전공학과) ;
  • 박용복 (경북대학교 자연과학대학 유전공학과) ;
  • 박용태 (경북대학교 자연과학대학 화학과) ;
  • 하지홍 (경북대학교 자연과학대학 유전공학과)
  • Published : 1995.02.01
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Abstract

During the screening of antifungal antibiotics from microbial metabolites, we selected Pseudomonas aeruginosa KGM-100 showing powerful antagonistic activity against various phytopathogenic fungi. Antibiotics KGM-100A and KGM-100B were purified from the culture broth of Pseudomonas aeruginosa KGM-100 by diaion HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, preparative TLC and recrystallization. KGM-100A which was recrystallized in MeOH showed antimicrobial activities against a broad spectrum of fungi and bacteria. Physico-chemical properties of KGM-100A were determined and identified to be phenazine-l-carboxylic acid by UV, IR, $^{1}$H-NMR, $^{13}$C-NMR, mass spectrum, and elemental analyses.

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