Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Properties of $\beta$-1,3-glucan Synthase System in Saccharomyces cerevisiae

Saccharomyces cerevisiae의 베타-1,3-글루칸 합성효소 체계의 특성

  • 박희문 (충남대학교 미생물학과) ;
  • 김정윤 (충남대학교 미생물학과) ;
  • 김성욱 (KIST 유전공학연구소 생물소재그룹) ;
  • 복성해 (KIST 유전공학연구소 생물소재그룹)
  • Published : 1995.06.01
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Abstract

Some properties of $\beta$-1, 3-glucan synthase system in Saccharamyces cerevisiae were investigated. By extraction with detergent and salt, the membrane preparations could be dissociated into two components, one soluble, the other still membrane bound. Both components, in addition to GTP, were necessary for the activity of $\beta$-1, 3-glucan synthase like other fungi. The protective effect of guanosine nucleotides on the soluble factor pointed to the possibility that this fraction contained a GTP-binding protein. Addition of increasing amounts of soluble factor to a constant amount of insoluble catalytic factor, vice versa, gave rise to a saturation curve. These results, including different types of evidence, indicate that the soluble factor and the catalytic factor form a complex.

Keywords

References

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