한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제23권3호
  • /
  • Pages.311-315
  • /
  • 1995
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

베타-1,3-글루칸 합성효소 저해제의 스크리닝을 위한 효소원 제조법

Preparation of Enzyme Source for Screening of Enzyme Inhibitor of $\beta$-1,3-glucan Synthase

  • 박희문 (충남대학교 미생물학과) ;
  • 이동원 (충남대학교 미생물학과) ;
  • 송미령 (충남대학교 미생물학과) ;
  • 김정윤 (충남대학교 미생물학과) ;
  • 김성욱 (KIST 유전공학연구소 생물소재그룹) ;
  • 복성해 (KIST 유전공학연구소 생물소재그룹)
  • 발행 : 1995.06.01
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초록

Assay conditions for screening of $\beta$-1,3-glucan synthase inhibitor were evaluated. Cells in the beginning of mid-log phase showed the highest activity of the $\beta$-1,3-glucan synthase. Cells permeabilized with 1% digitonin treatment could be used as a good crude enzyme source for convenient screening of the $\beta$-1,3-glucan synthase inhibitors. Calcofluor white (0.125% in final) and papulacandin B (25 $\mu$g/ml) inhibit 90% and more than 50% of the $\beta$-1,3-glucan synthase activity, respectively. Cells grown at 37$\circ$C showed higher enzyme activity than those of 25$\circ$C. Catalytic factor of the $\beta$-1,3-glucan synthase was solubilized from particulated membrane preparations, holoenzyme, by extracting with 0.00938% CHAPS.

키워드

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