소량의 Bifidobacterium 배양액에서 genomic DNA 추출을 위한 신속/간단한 방법

A Micromethod for Rapid and Simple Isolation of Genomic DNA from Small Scale Culture of Bifidobacterium

  • 제갈수 (인하대학교 생물공학과) ;
  • 박희경 (인하대학교 생물공학과) ;
  • 송지은 (인하대학교 생물공학과) ;
  • 허태련 (인하대학교 생물공학과) ;
  • 소재성 (인하대학교 생물공학과)
  • 발행 : 1995.12.01

초록

A method is described for the rapid and simple isolation of genomic DNA from 3 ml culture of Bifidobacterium. The method is expected to be used in gene manipulation of Bifidobacterium spp. The isolated DNA using this method is shown to be an excellent substrate for restriction endonuclease digestion and ligation with T4 DNA ligase.

키워드

참고문헌

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