한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제23권6호
  • /
  • Pages.781-783
  • /
  • 1995
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

소량의 Bifidobacterium 배양액에서 genomic DNA 추출을 위한 신속/간단한 방법

A Micromethod for Rapid and Simple Isolation of Genomic DNA from Small Scale Culture of Bifidobacterium

  • 제갈수 (인하대학교 생물공학과) ;
  • 박희경 (인하대학교 생물공학과) ;
  • 송지은 (인하대학교 생물공학과) ;
  • 허태련 (인하대학교 생물공학과) ;
  • 소재성 (인하대학교 생물공학과)
  • 발행 : 1995.12.01
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초록

A method is described for the rapid and simple isolation of genomic DNA from 3 ml culture of Bifidobacterium. The method is expected to be used in gene manipulation of Bifidobacterium spp. The isolated DNA using this method is shown to be an excellent substrate for restriction endonuclease digestion and ligation with T4 DNA ligase.

키워드

참고문헌

  1. Bifidobacteria and their role Rasic,J.;J.Kurman
  2. J. Dairy Sci. v.73 Growth of Bifidobacteria and their enzyme profiles Marie-Line Desjardins;Densi Roy
  3. Gene Cloning An Introduction T.A.Brown
  4. J. Dairy Sci. v.67 Growth of bifidobacteria in milk and preparation of Bifidobacterium infantis for a dietary adjunct E.B.Collins;B.J.Hall
  5. Appl. Environ. Microbiol. v.59 Rapid Mini-Prep Isolation of High-Quality Plasmid DNA from Lactococcus and Lactobacillus spp Daniel J.O' Sullivan ;Todd R. Klaenhammer
  6. A laboratory manual. Molechular Clong. Sambrook,J.;E.F.Fritsch;T.Maniatis
  7. J. of Microbiol. Methods v.17 Improved lysis of wine lactobacilli for high yield isolation and characterzation of chromosomal DNA J.B.Velazquez;J.Cansado;C.Sieiro;P.Calo;E.Longo;T.G.Villa
  8. Can. J. Microbiol. v.36 An improved method for rapid isolation of chromosomal DNA from Mycoplasma spp Patricia G. Hempstead