Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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In situ Separation of Lactic Acid by Electrodialysis in Batch Culture

회분식 발효에서의 전기투석에 의한 젖산의 동시분리

  • 이은교 (한국과학기술원 화학공학과 및 생물공정연구센터) ;
  • 장용근 (한국과학기술원 화학공학과 및 생물공정연구센터) ;
  • 장호남 (한국과학기술원 화학공학과 및 생물공정연구센터) ;
  • 김인호 (충남대학교 화학공학과)
  • Published : 1995.10.01
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Abstract

Effects of lactate concentration, temperature, counter ions, pH as well as voltage (current) in batch electrodialysis (ED) experiments with a 3-compartment unit were investigated. The applied voltage was found to be the most critical factor as expected. The electrodialysis rate increased with the lactate concentration of the source solution. The amount of lactate transferred was limited by the lactate concentration difference between cathode and permeate compartments. The electrodialysis rate did not heavily depend on temperature change. The electrodialysis rate of NH$_{4}$-lactate was faster than that of Na-lactate and both lactates showed the highest electrodialysis rate at a pH of 4.0. A little amount of non-ionic glucose diffused through the anionic membrane to the permeate compartment. To test the effectiveness of the in situ recovery of lactic acid from fermentation broth by ED, three cases of batch culture were carried out; pH control only, ED only, and pH control and ED. The case with both pH control and ED was more efficient than that with pH control only in the aspects of productivity and product yield.

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References

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