Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Isolation and Characterization of a Cephalosporin C Resistant and 7-Aminocephalosporanic Acid Sensitive Strain

Cephalosporin C 내성과 7-Aminocephalosporanic Acid 감수성을 지닌 균주의 선발 및 특성

  • 김욱현 (충북대학교 자연과학대학 미생물학과) ;
  • 박용춘 (충북대학교 자연과학대학 미생물학과) ;
  • 임재윤 (충북대학교 자연과학대학 미생물학과) ;
  • 김영창 (충북대학교 자연과학대학 미생물학과)
  • Published : 1995.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

A strain which showed cephalosporin C resistance and 7-aminocephalosporanic acid sensitivity was isolated from nature. Among the isolates, SS5 was sensitive to cephalosporin C, penicillin G, ampicillin, 7-aminocephalosporanic acid, 6-aminopenicillanic acid, and 7-aminodeacetoxy cephatosporanic acid at concentrations of 1,000 $\mu $g/ml, 2,000 $\mu $g/ml, 3,000 $\mu $g/ml, 30 $\mu $g/ml 100 $\mu $g/ml and 100 $\mu $g/ml, respectively. But SS5 was sensitive at very low concentration of chloramphenicol, kanamycin, neomycin, streptomycin and tetracycline. Since SS5 was sensitive to 7-ACA (30 $\mu $g/ml) and didn't have $\beta $-lactamase activity on the cephalosporin C, SS5 could be useful as an indicator strain for the production of 7-ACA, which is an important precursor for the synthesis of many semisynthetic cephalosporins.

Keywords

References

  1. Biochem. J. v.79 The structure of cephalosporin C Abraham,E.P.;G.F.Newton
  2. J. Ferment. Bioeng. v.72 Isolation of soil strain producing new cephalosporin acylase Aramori,I.;M.Fukagawa;M.Tsumura;M.Iwami;Y.Yokota;H.Kojo;M.Kohsaka;Y.Ueda;H.Imanaka
  3. J. Med. Chem. v.27 Substituent ettect on reactivity and spectral paramaetrs of cephalosporins Coene,B.;A.Schanck;J.M.Dereppe;M.V.Meerssche
  4. Biochem. J. v.79 The cephalosporin C nucleus(7-aminocephalosporanic acid) and some of its derevatives Loder,B.;G.F.Newtion;E.P.Abraham
  5. J. Bacteriol. v.169 Cloning and characterization of the genes for two distinct cephalosporin acylase from a Pseudomonas strain Matsuda,A.;K.Matsuyama;K.Yamamoto;S.Ichikawa;K.Komatsu
  6. Enzyme Engineering v.5 Cloning of the penicillin G acylase gene of Escherichia coli ATCC 11105 on multicopy plasmids Mayer,H.;J.Collins;F.Wagner
  7. J. Med. Chem. v.27 3-Substituent effect and 3-methylene substituent effect on the structure-reactivity of 7β-(acylamino)-3-cephem-4-carboxylic acid derivatives studies by carbon-13 and IR spectroscopies Nishikawa,J.;K.Tori
  8. Agric. Biol. Chem. v.45 Isolation and properties of 7β-(4-carboxybutanamido)cephalosporanic acid acylase-producing bacteria Shibuya,Y.;K.Matsumoto;T.Fujii
  9. J. Am. Chem. Soc, v.94 Enzymatic synthesis of cephalosporins Takahashi,T.;Y.Yamazaki;K.Kato;M.Isono
  10. J. Antibiot. v.33 Cephalosporin derivatives in the furyl properties Tinti,M.O.;P.Foresta;E.Quaresima;P.De Witt;M.T.Ramacci
  11. Agric. Biol. Chem. v.45 Isolation and properties of 7β-(4-carboxybutanamido)cephalosproanic acid acylase producing bacteria Yuzo,S.;M.Kunio;T.Fujii