한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제23권5호
  • /
  • Pages.531-535
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  • 1995
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Pseudomonas sp. CH-414에 의한 Eicosapentaenoic Acid 생산

Production of Eicosapentaenoic Acid by Pseudomonas sp. CH-414

  • 김창호 (강원대학교 발효공학과 및 생물산업소재연구센터) ;
  • 홍억기 (강원대학교 발효공학과 및 생물산업소재연구센터) ;
  • 신원철 (강원대학교 발효공학과 및 생물산업소재연구센터)
  • 발행 : 1995.10.01
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초록

The identification of eicosapentaenoic acid (EPA) by Pseudomonas sp. CH-414 were investigated under the optimal culture conditions. The maximum dry cell weight and phospholipid production showed about 10 mg/ml and 0.6 mg/ml, respectively, at the 48 hr cultivation. The phospholipid form produced by Pseudomonas sp. CH-414 was elucidated as a phosphatidyt ethanolamine by thin layer chromatography. EPA was contained about 15% in the. extractable lipid, and the amount of EPA was about 83 $\mu $g/ml from the culture broth. Also myristic, palmitic, palmitoleic, stearic and oleic acids were identified with gas chromatography.

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