한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제23권5호
  • /
  • Pages.499-505
  • /
  • 1995
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

다공성 젤라틴 미립담체를 이용한 동물세포의 배양시 담체 내외부의 세포 분포 및 전단응력의 영향

Cell Distribution and Shear Effect on Mammalian Cell Growth in Macroporous Gelatin Microcarriers

  • 임현수 (한국과학기술원 생물공학과) ;
  • 김정회 (한국과학기술원 생물공학과)
  • 발행 : 1995.10.01
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초록

Immobilization of anchorage-dependent animal cells was investigated using macroporous gelatin microcarriers developed in our laboratory. For the observation of the distribution of cells in macroporous beads, Vero-6 cells and CHO cells were cuttured and their distribution in macroporous beads was observed using a confocal microscope. In results, the final concentration of Vero-6 cells and CHO cells on macroporous beads was 2-3 times higher than that on commercial solid microcarriers (Cytodex-3). Also, macroporous microcarriers could hold cells in their macropores. Consequently, the pores protected cells against hydrodynamic shear. Based on Kolmogorov eddy length scale, the smaller eddies (80 $\mu $m) showed the detrimental effect on cells in macroporous beads as compared with 160 $\mu $m of eddies in conventional solid microcarriers.

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참고문헌

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