KSBB Journal
- Volume 10 Issue 5
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- Pages.582-588
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- 1995
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- 1225-7117(pISSN)
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- 2288-8268(eISSN)
Purification and Characterizationn of Biosurfactant from Marine Pseudomonas sp. CHCS-2
해양으로부터 분리한 Pseudomonas sp. CHCS-2가 생산하는 Biosurfactant의 정제 및 특성에 관한 연구
Abstract
A marine microorganism producing biosurfactant was isolated from the oil polluted coast of Chung-Mu in Korea, and was identified as Pseudomonas sp.. It produced the biosurfactanl and its optimum culture conditions for pH and salt concentration were 8.0 and 3.0%, respectively. The productivity of biosurfactant from this strain was affected by the nitrogen source used. For the oil resolvability of the biosurfactant, the residual oil in the culture broth with 2% Kuwait crude oil at each time of 48, 96, and 132hr was investigated by gas chromatography. As result of this experiment, it was verified that the biosurfactant acted on C10-C14, of Kuwait crude oil and so the oil was decomposed. The biosurfactant isolated from the supernatant was purified by adsorption to Amberliter XAD-7 and followed by gel chromatography (Sephadex G-100) and HPLC. The purified biosurfactant showed a high value of emulsifying activity at
해양의 유류유출이 잦은 지역으로부터 crude oil 분해능이 뛰어난 미생물을 분리하여 통정한 결과 Pseudomoans 속으로 판명되였으며, 이를 Pseud
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