Growth and Differentiation of Mammary Epithelial Cells in Extracellular Maxtrix Culture

세포외 기질 세포 배양법에 의한 유선상피세포의 성장 및 분화 유도

  • 백기주 (부산대학교 약학대학 약학과) ;
  • 윤정현 (부산대학교 약학대학 약학과) ;
  • 김동염 (부산대학교 약학대학 약학과) ;
  • 전성실 (부산대학교 약학대학 약학과) ;
  • 양한석 (부산대학교 약학대학 약학과) ;
  • 김남득 (부산대학교 약학대학 약학과)
  • Published : 1995.02.01

Abstract

Mammary orgamoids(ductal and endbud fragments) were cultured in a complete hormone medium(CHM) with 10%FBS, estradiol, progesterone, hydrocortisone, insulin, and prolactin, Several types of colonies were observed: stellate(14$$\pm$5.5%), duct(41$\pm$5.6%), web(35$\pm$3.6%), squamous(6$\pm$2.1%), and lobuloduct(4$\pm$1.2%), Squamous colony was typical squamous metaplasia(SM) with several layers of squamous epithlia and keratin pearls. At the immunocytochemical study, casein proteins were predominantly localized near the apical surfaces of the cells or in the lumina of ductal or lobuloductal colonies. To inhibit the formation of SM, we treated organoids with all-trans retinoic acid(RA) from 10$^{-6}$ to 10$^{-17}$ M in CHM. Formation of SN was completely inhibited at 10$^{-9}$M RA in CHM. The frequency of lobuloductal colony formation was increased with the augmentation of RA concentration.

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