Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Cloning of the gense coding for extracellular proteases from alkalophilic xanthomonas SP. JK311

  • Kim, Young-Hun (Department of Biology, Inje University) ;
  • Jang, Ji-Yeon (Department of Biology, Inje University) ;
  • Yeehn Yeeh (Department of Institute of Basic Science, Inje University) ;
  • Kim, Yong-Ho (Department of Medical Laboratory Science, Inje University) ;
  • Kim, Sang-Hae (Department of Biology, Inje University)
  • Published : 1995.12.01
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Abstract

The alkalophilic bacterium, Xanthomonas sp. JK311, producing extracellular proteases, was isolated from soil. Xanthomonas sp. JK311 produced five extracellular proteases that are all metalloproteases. Four of them were resistant against 1% SDS. Chromosomal DNA of the Xanthomonas sp. JK311 was digested with BamHI and cloned into PUC19. Among E. coli strain HB101 transformants, a clone secreting the proteases was screened through halo formation on skim-milk agar plate and by Southern blot analysis. It had the recombinant plasmid pXEP-1 containing the 7.5 kb-BamHI DNA fragment and produced three extacellular proteases. Their protease properties corresponded to those of Xanthomonas sp. JK311.

Keywords

References

  1. Appl. Biochem. & Bioengin. v.2 Production, isolation, and economics of extracellular enzymes Aunstrup,K.
  2. J. Gen. Microbiol. v.133 Cloning, expression and release of a Vibrio alginolyticus SDS-resistant Ca -dependent exoprotease in Escherichia coli Deane,S.M.;J.R.Mahara;F.T.Robb;D.R.Wood
  3. J. Gen. Microbiol. v.132 Isolation and characterization of a Vibrio alginolyticus mutant that overproduces extracellular proteases Deane,S.M.;F.T.Robb;D.R.Wood
  4. J. Gen. Microbiol. v.133 Production and activation of a SDS-resistant alkaline serine exoprotease of V. alginolyticus Deane,S.M.;F.T.Robb;D.R.Wood
  5. Geve v.76 Nucleotide sequence of the Vibrio alginolyticus calcium-dependent, detergent-resistant alkaline serine exoprotease A Deane,S.M.;F.T.Robb;S.M.Robb;D.R.Wood
  6. J. Bacteriol. v.171 Structural gene and complete amino acid sequence of Pseudomonas aeraginosa IFO 3455 elastase Fukushima,J.;S.Yamamoto;K.Morihara;Y.Atsumi;H.Takeuchi;S.Kawamoto;K.Okuda
  7. FEBS Lett v.199 Amino acid sequence of proteinase K from the mold Tritrirachium album Limber Jany,K.D.;G.Lederer;B.Mayer
  8. Gene v.125 Cloning, expression and sequencing of the minor protease encoding gene from Serratia marcescens ATCC 21074 Kwon,Y.T.;H.H.Lee;H.M.Rho
  9. Subtilisins: Primary structure and physical properties In The enzymes Markland,F.S.;E.L.Smith;P.D.Boyer(ed.)
  10. Agric. Biol. Chem. v.34 Serratia protease: Purification and general properties of the enzyme Miyata,K.;K.Maejima;K.Tomoda;M.Isono
  11. Bacteriol. Rev. v.41 Extracellular enzyme synthesis in the genus Bacillus Priest,F.G.
  12. J. Bacteriol. v.161 Cell bound and secreted protease of Serratia marcescens Schmitz,G.;V.Braun
  13. J. Mol. Biol. v.98 Detection of specific sequences among DNA fragment separated by gel electrophoresis Southern,E.M.
  14. Appl. Microbiol. v.28 Growth and reproduction of microorganisms under extremely alkaline conditions Souza,K.A.;P.H.Deal;H.M.Mack;C.E.Turnbill
  15. J. Bacteriol. v.163 Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus Takagi,M.;T.Imanaka;S.Aide
  16. J. Biol. Chem. v.260 The OmpA signal peptide directed secretion of staphylococcal nuclease A by Escherichia coli Takahara,M.;D.W.Hibleer;P.J.Barr;J.A.Gerlt;M.Inouye
  17. Prokaryotic life in the alkaline, saline, athalassic environment In Halophilic Bacteria, CRC, 1 Tindall,B.J.;F.Rodriguez-valera(ed.)
  18. J. Bacteriol. v.160 Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro derived deletion mutation Yang,M.Y.;E.Ferrari;D.J.Henner
  19. J. Bacteriol. v.166 Specific excretion of Serratia marcescens protease through the outer membrane of Escherichia coli Yanagida,N.;T.Uozumi;T.Beppu