Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Identification of hemolysin as one of the important virulent factors in vibrio anguillarum V7

  • Choe, Young-Chool (Research Center for Molecular Microbiology, Seoul National University) ;
  • Jeong, Gajin (Research Center for Molecular Microbiology, Seoul National University)
  • Published : 1995.12.01
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Abstract

We have identified hemolysin rendering virulency of Vibrio anguilarum grown at 23.deg.C which was evaluated on human RBCs. Hemolysin itself was separated as a single band on non-denaturing gel electrophoresis. Vibrio hemolysin was destroyed by trypsin and proteinase K and was heat labile. Optimal pH for activity was around pH 6 while pI of the molecule was recognized as 5.7, with relative distance (R$\sub$f/) on non-denaturing gel was 0.7. Addition of EDTA and FeCI$\sub$3/ drew the possibility that the production of hemolysin was mainly induced to overcome iron deficiency inside host animalsd infection.

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References

  1. Am. Fish Soc. Publ. v.5 Vibrio disease in marine fishes Anderson,J.I.W.;D.A.Conroy
  2. Zentralbl. Bakertiol. Parasitenkd. Infektionskr. Hyg. Abt. 1. Orig. v.62 Eineansteckende Augenkrankheit, Keratomalacie. bei Dorschen und der Sudkuste Schwedens Bergman,A.M.
  3. J. Bacteriol. v.152 Phospholipase C(geat-labile hemolysin) of Pseudomonas aeruginosa: Production and preliminary charcterization Berka,R.M.;M.L.Vasil
  4. J. Med. Micribiol. v.15 Cytotoxic activity of partially purified Escherichi coli alpha-hemolysin Cavalieri,S.J.;I.S.Snyder
  5. Kor. Jour. Microbiol. v.32 Virulence evaluation of Vibrio anguillarum in accordance with hemolytic activity on human red blood cells Choe,Y.;G.Jeong
  6. Infect. Immun. v.18 Evidence for plasmid contribution to the virulence of the fish pathogen Vibrio anguillarum Crosa,J.H.;M.H.Schiewe;S.Falkaw
  7. Infect. Immun. v.27 Curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen Ⅴ Crosa,J.H.;L.L.Hodges;M.H.Schiewe
  8. Infect. Immun. v.31 Outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen Vibrio angullarum Crosa,J.H.;L.L.Hodges
  9. Ann. N.Y. Aca. Sci. v.121 Disk electrophoresisⅡ. Method acid application to human serum proteins Davis,B.J.
  10. J. Bacteriol. Rev. v.47 Lecithinase production in gram negative bacteria Esselman,M.;P.V.Liu
  11. J. Fish Res. Board Can. v.1 First records of vibriosis in Pacific salmon cultured in Canada and taxonomic status of the responsible bacterium, Vibrio anguillarum Evelyin,T.P.T.
  12. J. Biol. Chem. v.250 High resolution two-dimensional electrophoresis of proteins O'Farrell,P.H.
  13. J. Fish Biol. v.4 The occurrence of vibrio diseases in wild Norwegian fish Haastein,T.;G.Holt
  14. J. Wild. Dis. v.7 Vibrio infection in tropical fish in a freshwater aquarium Hacking,M.A.;J.Budd
  15. Fish Bull. U.S. v.74 Isolation and description of two vibrios pathogenic to Pacific salmon in Puget Sound, Washington Harrell,L.W.;A.J.Nowotny;M.H.Schiewe;H.O.Hodgins
  16. Fish Pathol. v.19 Toxicity of extacellular products of Vibrio anguilarum Inamura,H.;K.Muroga;T.Nakai
  17. Fish Pathol. v.29 Partial purification of extracellular substance of Vibrio anguillarum toxigenic for rainbow trout and mouse Kodama,H.;M.Moustafa;T.Mikami;H.Izawa
  18. Kor. Jour. Microbiol. v.32 Characterization of the nature of virulency caused by Vibrio anguillarum: Identification of an outer membrane protein rendering virulency Lee,J.S.;G.Jeong;Y.C.Hah
  19. Can J. Microbiol. v.18 Vibrio anguillarum as a cause of disease in winter flounder (Pseudopleuronectes americanus) Levine,M.A.;R.E.Wolke;V.J.Cambelli
  20. J. Med. Micribiol. v.15 The role of alpha-hemolysin in the virulence of Escherichia coli for mice Lingood,M.A.;P.L.ingram
  21. Nature (London) v.188 A filtrable hemolysin from Escherichia coli Lovell,R.;T.A.Rees
  22. Infect. Immun. v.57 Cloning and nucleotide sequence of the gene (trh) encoding the hemolysin related to the thermostable direct hemolysin of Vibrio parahemolyticus Nishibuchi,N.;T.Taniguchi;T.Misawa;V.Khaeomanee-Iam;T.Honda;T.Waton
  23. J. Immunol. v.126 The complement system of rainbow trout (Salmo gairdneri)Ⅰ. Identification of the serum lytic system homologous to mammalian complement Nonaka,M.;N.Yamaguchi;s.N.Saiki;M.Takahashi
  24. J. Med. Micribiol. v.7 The kinetics of erythrocyte lysis by Escherichia coli hemolysin Rennie,R.P.;J.H.Freer;J.P.Arbuthnott
  25. The bacteriology of teleosts In Fish Pathology Richards,R.H.;R.J.Roberts;R.J.Roberts(ed.)
  26. Prg. Fish Cult. v.21 Vibrio infection among marine and fresh water fish Rucker,R.R.
  27. Am. Fish. Soc. Publ. v.83 Infections diseases of Pacific salmon trians Rucker,R.R.;B.J.Earp;E.J.Ordal
  28. Infect. Immun. v.3 Properties of the hemolytic activity of Escherichia coli Short,E.C.Jr.;H.J.Kurtz
  29. J. Gen. Microbiol. v.24 A disease of finnock due to Vibirio anguillarum Smith,I.W.
  30. J. Bacteriol. v.144 Production and characterization of hemolysins of Eshcerichia coli Snyder,I.S.;N.A.Koch
  31. Infect. Immun. v.58 Effect of Escherichia coli hemolysin on the endothelial cell function Suttorp,N.;B.Floer;H.Schnittler;W.Seegers;S.Bhakdi
  32. Infect. Immun. v.42 In vivo function of hemolysin in the nephropathogenecity of Escherichia coli Waalwijk,C.;D.M.Maclaren;J.de Graff