Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Binding sites for lead ion in staphylococcus epidermidis

  • Kim, Mal-Nam (Department of Biology, Sangmyung Womens University) ;
  • Sung, Hye-Yoon (Department of Biology, Sangmyung Womens University)
  • Published : 1995.09.01
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Abstract

As S. epidermidis cell was fractionated into cell wall, cell membrane, and cytoplasm, the cell membrane proved to be the most efficient absorbent for lead ion. Utrasonication was effective, when the cells were treated during their exponential growth. The amount of the lead ion adsorbed in cell membrane decreased as hydrogen ion concentration of solution increased. Protein purified from the cell membrane showed higher adsorption capacity for the lead ion than peptidoglycan, teichoic acid from cell wall, or cell membrane lipid. Modification of carboxyl groups in the membrane protein with ethylenediamine and 1-ethyl-3-carbodiimide hydrochloride resulted in a considerable decrease of lead ion adsorption capability, suggesting that the main binding site for lead ion was the carboxyl groups of protein in cell membrane.

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