Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지

A Study on the metabolism mechanism of Benzene, Toluene and Xylene by Cytochrome P-450 dependent radical-mediated

Cytochrome P-450 의존성 radical 전달에 의한 Benzene, Toluene, Xylene의 대사기전 연구

  • 김기웅 (한국산업안전공단 산업보건연구원) ;
  • 장성근 (순천향대학교 자연대학 화학과) ;
  • 김양호 (한국산업안전공단 산업보건연구원) ;
  • 문영한 (한국산업안전공단 산업보건연구원)
  • Published : 1995.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was undertaken to investigate the effects of organic solvents on xenobiotic metabollzing enzyme system in vivo by meaas of experimental conditions i.e. (1) single group which was treated by benzene (B), toluene (T) and xylene (X), respectively, (2) combination group which was treated by mixture of benzene+toluene (BT), benzene+xylene (BX), and toluene+xylene (TX), respectively, (3) mixture group which was treated by benzene+ toluene+xylene mixture (M), and to interpreat the interaction between the organic solvents metabolizing enzymes. 1. The contents of cytochrome P-450 in liver microsomes were increased (p < 0.01) in organic solvents treated groups, and the contents of cytochrome P-450 were increased by following order of B < T < M < BT=BX < X < TX. 2. The activity of cytochrome P-450 dependent AHHase was significantly higher in organic solvents treated groups than in control group (p < 0.01), and the activity of AHHase was increased by following order of B < T < BT=BX=TX=xylene < M. 3. The activity of NADPH P-450 reductase was significantly higher in organic solvents treated groups than in control group (p < 0.01), and the order of M < combinated group < X < T

Keywords

References

  1. 예방의학회지 v.21 Ethanol이 trichloroethylene 대사효소의 활성도와 유도성에 미치는 영향 김기웅:강성규;최병순;조영숙;이세휘;문영한;박상신
  2. Trichloroethylene 처리한 흰쥐의 이물질 대사효소의 활성도에 관한 연구 김기웅;강성규;최병순;이종성;김종성;문영한
  3. 생화학총설집 v.1 박기현
  4. 한국노화학회지 v.3 알킨산 첨가 요구르트가 흰쥐의 비만억제와 생리작용에 미치는 영향 최진호;김재일;김동우;오두환
  5. J. Pharmacology. Ther. v.204 Metabolic activation of trichloroethylene into a chemically reactive metabolite toxic to the liver Allemand,H.;Pesayer,D.;Descatoire,V.;Degott,C.;Fildmann,G.;Benhamou,J.P.
  6. Environ, Mol. Mutagen. v.14 Mutagensis and carcinogenesis Ames,B.N.
  7. Arch. Toxicol. v.65 Stimulatory effects of benzene on rabbit liver and kidney microsomal cytochrome P-405 dependent drug metabolizaing enzymes Arinc,E.;Adali,O.;Iscan,M.;Guray,T.
  8. Solvent neurotoxicty Arlien Soborg,P.
  9. Xenobiotica v.25 N-hydroxylation and Ndealkylation by P4502C3 of N-methyl- benzamidin. N-oxygenation and N-oxydative dealkylation of one functional group Clement,B.;Jung,F.
  10. Basic environmental toxicology Cockerham,L.G.;Shane,B.S.
  11. Scand. J. Work. Environ. Health. v.5 Toluene. a toxicology review Cohr,K.H.;Stokholm,J.
  12. Mutation. Res. v.47 Genetic toxicology of benzene, toluene, xylene and phenol Dean,B.J.
  13. Mutation. Res. v.154 Recent findings on the genetic toxicology of benzene, toluene, xylene and phenols Dean,B.J.
  14. pathology of oxygen Oxygen free radicals in central nervous ischemia and trauma Demopoulos,H.B.;Flamm,E.;Seligman,M.;Pietronigro,D.D.;ed.Autor,A.P.
  15. Cancer. Res. v.29 A microsome-dependent binding of benzo(a)pyrene to DNA Gelboin,H.V.
  16. Chem. Res. Toxicol. v.4 Role of human cytochrome P-450IIE1 in the oxidation of many low molecular weight cancer suspects Guengerich,F.P.;Kim,D.H.;Iwasaki,M.
  17. Mol. Aspects. Med. v.8 The importance of free radicals and catalytic metal ions in human diseases Halliwell,B.;Gutteridge,J.M.C.
  18. Methods in Enzymol. v.52 Methemoglobin reduction system of erythrocytes Hultquist,D.E.
  19. Xenobiotica v.12 On the significance of the cytochrome P-405 dependent hydroxy radical-mediated oxygenation mechanism Ingelman Sundberg,M.;Hagbjork,A.L.
  20. Biochem. Pharmacol. v.32 A high spin form of cytochrome P-448 highly purified from PCB-treated rat II. Characteristic requirement of cytochrome b5 for maximun activity Kamataki,T.;Maeda,K.;Ishil,K.;Yamazoe,Y.;Kato,R.
  21. Chem. Res. Toxicol. v.4 The role of oxidative damage in metal carcinogenicity Kasprzak,K.S.
  22. Xenobiotica v.25 Three-dimensional models of human and other mammalian microsomal P450s constructed from an alignment with P450102 (P450) Lewis,D.F.V.
  23. J. Biol. Chem. v.193 Protein measurement with folin phenol reagent Lowry,O.H.;Rosebrough,N.J.;Farr,A.L.;Randall,R.J.
  24. Pharmacol. Rew. v.31 Multiplicity of mammalian microsomal cytochromes P-450 Lu,A.Y.H.;West,S.B.
  25. Methods in Enzymol. v.10 The preparation and properties of microsomal TPNH-cytochrome C reductase from pig liver Master,B.S.S.;Williams,C.H.Jr;Kamin,H.
  26. Biochemistry v.21 Oxidation of trichloroethylene by liver microsomal cytochrome P-450. Evidence for chlorine migration in a transition state not involving trichloroethylene oxide Miller,R.E.;Guengerich,F.P.
  27. Br. J. Ind. Med. v.47 Exposure to toluene increses the uninary excretion of D-glucaric acid Moretto,A.;Lotti,M.
  28. Toxicol. Appl. Pharmacol. v.94 Ethanol-induced Enhancement of Trichloroethylene Metabolism and hepatotoxicity: Difference from the Effect of Phenobarbital Nakajima,T.;Okino,T.;Okuyama,S.;Kaneko,T.;Yonekura,I.Sato.A.
  29. Biochem. Pharmacol. v.43 A comparative study on the contribution of cytochrome _0450 isozymes to metabolism of benzene, toluene and trichloroethylene in rat liver Nakajima,T.;Wang,R.S.;Elovara,E.;Park,S.S.;Gelboin,H.V.;Varinio,H.
  30. Toxicol. Appl. Pharmacol. v.102 Three forms of trichloroethylene metabolizing enzymes in rat liver induced by ethanol, phenobarbital, and 3-methy1-cholanthrene Nakajima,T.;Wang,R.S.;Murayama,N.;Sato,A.
  31. J. Biol. Chem. v.234 Substrate inducible microsomal ary1 hydrocarbon hydroxylase in mammalian cell culture. I. Assay and properties of the induced enzymes Nebert,D.W.;Gelboin,H.V.
  32. Ann. Rev. Biochem. v.56 P450 Gene. Structure, evoluation and regulation Nebert,D.W.;Gonzalez,F.J.
  33. Br. J. Ind. Med. v.27 Urinary excretion of hippuric acid and m- or p-methylhippuric acid in th eurine of persons exposed to vapours of toluene and m-or-p-xyleneas a test of exposure Ogata,M.;Tomokuni,K.;Takatsuka,Y.
  34. Toxicol. Appl. Pharamacol. v.108 Morphological and biochemical analyses of trichloroethylene hepatotoxicy. Differences in ethanol-, and phenobarbital-pretreated rats Okino,T.;Nakajima,T.;Nakano,M.
  35. Br. J. Cancer. v.55 The formation, identification, and significance of DNA-protein cross-links in mammalian cells Oleinick,N.L.;Chiu,S.;Ramakrishnan,N.;Xue,L.
  36. J. Biol. Chem. v.239 The carbon monooxide binding pigment of liver microsomes. I. Evidence for its hemoprtein nature Omura,T.;Sato,R.
  37. Drug. Meta. Rew. v.27 Inhibition of P450 cytochromes by reactive intermediates Osawa,Y.;Davila,J.C.;Nakatsuka,M.;Meyer,C.A.;Darbyshire,J.F.
  38. Anal. Biochem. v.42 Reevaluation of assay methods and establishment of kit for superoxide dismutase activity Oyanagui,Y.
  39. Biochem. J. v.54 Studies in detoxication. The metabolism of benzene containing 14C-benzene Park,D.V.;William.R.T.
  40. Korean Biochem. J. v.17 Induction of the differenct forms cytochrome P-450 isozymes and comparison of aryl hydrocarbon hydroxylase levels on rat tissues by chemical treatment Park,K.H.;Kim,C.R.
  41. Toxicol. Appl. Pharmacol. v.82 A comparative study of the effects of benzene, toluene, and xylenes on their in vivo metabolism and durg-metabolizing enzymes in rat liver Pathiratne,A.;Puyear,R.L.;Brammer,J.D.
  42. Chem. Biol. Interact. v.44 Time course of effects of toluene on microsomal enzymes in rat liver, kidney and lung during and after inhalation exposure Pyykko,K.
  43. Environ. Health. Perspect. v.21 Damage to hepatic cellular membranes by chlorinated olefins with emphasis on synergism and antagonism Reynold,E.S.;Moslen,M.T.
  44. Am. J. Ind. Med. v.11 Hippuric acid and ortho-cresol as biological indicators of occupational exposure to toluene Rosa,E.D.;Bartolucci,G.B.;Sigon,M.;Callegaro,R.;Perbellini,L.Brugnone,F.
  45. Toxicol. Appl. Pharmacol. v.99 Effect of exposure concentrations, exposure rats. and route of administration on metabolism of benzene by F 344 rats and B6C3F1 mice Sabourin,P.J.;Bechtold,W.E.;Griffith,W.C.;Birnbaum,L.S.;Lucier,G.;Henderson,R.F.
  46. Toxicology v.23 Effects of xylene and sylene isomers on cytochrome P-450 and invitro enzymatic activities in rat liver, kidney and lung Toftgard,R.;Nilsen,O.G.
  47. Xenobiotica v.25 Small intestinal sulphoxidation of Albendazole Villaverde,C.;Alvarez,A.L.;Redondo,P.;Voces,J.;Del Estal,J.L.;Prieto,J.G.
  48. Chem. Physics. Lipids v.45 Lipid peroxide and human diseases Yagi,K.
  49. Xenobiotica v.25 Relationship between hydrocarbon structure and induction of P450 effect on RNA levels Yuan,W.;White,T.B.;White,J.W.;Strobel,H.W.;Backes,W.L.