Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Neuronal Cytotoxicity of Oxygen Radical in Newborn Mouse Forebrain Culture

  • Lim, Kye-Taek (Department of Animal Science, Chonnam National University) ;
  • Park, Seung-Taeck (Department of Anatomy, Wonkwang University School of Medicine) ;
  • Choi, Min-Kyu (Department of Anatomy, Wonkwang University School of Medicine) ;
  • Chung, Yeun-Tai (Department of Anatomy, Wonkwang University School of Medicine)
  • Published : 1995.12.01
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Abstract

The cytotoxic effects of hydrogen peroxide and neuroprotective effects of a variety of agents were investigated in newborn mouse forebrain tissue culture. In our experiments, oxygen radical was generated enzymatically by glucose oxidase and the values were expressed as a percentage of number of living cells by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Cytotoxicity of oxygen radicals was prevented by catalase and (N, N, N', N', -tetrakis (2-pyridylmethyl) ethylenediamine (TPEN), but N-tetra-ot-butyl-phenylnitrone (PBN), and deferoxamine (DFX), failed to show protective effects against oxygen radicals. Antagonists of the N-methyl-D-aspartate (NMDA) receptor, D-2-amino-5-phosphonovaleric acid (APV), 7-chlorokynurenic acid (CKA), and MK801 (a non-competitive NMDA antagonist) were also not effective in blocking neurotoxicity induced by glucose oxidase generated oxygen radicals.

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References

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