Journal of Chest Surgery

  • 제28권8호
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  • Pages.731-741
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  • 1995
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  • 2765-1606(pISSN)
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  • 2765-1614(eISSN)
대한심장혈관흉부외과학회 (The Korean Society for Thoracic and Cardiovascular Surgery)
권호 표지

토끼의 대동맥 및 폐동맥 판막 동종이식편의 냉장 및 냉동 보존후 생육성 평가(I)

Viability Assay after $4^{\circ}C$ Cold Preservation & Cryopreservation of Aortic & Pulmonic Allograft Valves in Rabbits

  • 발행 : 1995.08.01
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초록

Cardiac valve allografts have been used as replacements for diseased valves and right ventricular outflow tract reconstruction, the long term follow-up of which has been reported satisfactory. For a good long-term result, it is essential that the allograft be viable at implantation. In this study, we aimed at preparing the cardiac valve allografts aseptically, preserving them at cold- and cryo-conditions, and testing the viability of the allografts after preservation by four methods. We tested the viability of the cardiac valve allografts preserved in cold refrigerated state[4$^{\circ}$C in nutrient media & in liquid nitrogen tank[cryopreservation under -149$^{\circ}$C for pre-planned time periods. The testing methods were 1 glucose utility test 2 tissue culture 3 thymidine uptake test and 4 histologic evidence by light microscopy. We observed no differences in the viability between cold- & cryo-groups and similar results among the methods for testing the viability. In conclusion, there was no difference in the viability between cold- and cryopreserved-allografts at least for 14 days of preservation. And glucose utility test and thymidine uptake test were satisfactory in the evaluation of the allograft viability, since they were easy and rapid with relatively quantitative results.

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참고문헌

  1. Ann. Thorac Surg. v.44 Intermediate-fate of cryopreserved allograft and xenograft valved conduits Kirklin,J.W.;Blackstone,E.H.;Maehara,T.(et al.)
  2. J. Thorac Cardiovasc. Surg. v.94 A comparison of aortic valve replacement with viable cryopreserved and fresh allograft valves O'Brien,M.F.;Stafford,E.G.;Gardner,M.A.H.;Pohlner,P.G.;McGiffin,D.C.;Kilklin,J.W.
  3. J. Cardiac. Surg. v.3 no.SUP. Long-term results of the viable cryopreserved allograft aortic valve : Continuing evidence for superior valve durability McGiffin,D.C.;O'Brien,M.F.;Stafford,E.G.;Gardner,M.A.;Pohlner,P.G.
  4. J. Cardiac. Surg. v.3 no.SUP. Pulmonary valve autotransplantation Ross,D.
  5. 세종의학 v.4 대동맥 동종이식 송명근
  6. 대흉외지 v.27 동종 동맥판의 생육석 평가에 관한 연구 (Ⅰ) 임창영;최영숙;홍은경;김종배
  7. Pathology v.15 Disinfection of human heart valve allografts with antibiotics in low concentration Stricett,M.G.;Barratt-Boyes,B.G.;MacCulloch,D.
  8. J. Cardiac. Surg. v.2 no.SUP. Measurement of postcryopreservation viability Blockbank,K.G.M.;Bank,H.L.
  9. Thorax v.30 Effect of preservation on the elasticity of human aortic valve homografts Ng,Y.L.;Wright,J.E.C.
  10. J. Surg. Res. v.30 Preservation of aortic heart valves with maintenance of cell viability Van der Kamp,A.W.M;Visser.W.J.;van Dongen,J.M.(et al.)
  11. Ann. Thorac Surg. v.21 Establishment of viable homograft cardiac valve bank: a rapid method of determining homograft viability Watts,L.K.;Duffy,P.M.B.;Field,R.B.;Stafford,E.G.;O'Brien,M.F.
  12. J. Cardiac. Surg. v.2 no.SUP. The viable cryopreserved allograft aortic valve O'Brien,M.F.;Stafford,G.;Gardner,M.(et al.)
  13. J. Cardiac. Surg. v.3 no.SUP. A study of the cells in the explanted viable cryopreserved allograft valve O'Brien,M.F.;Johnston,N.;Stafford,G.(ed al.)
  14. J. Thorac Cardiovasc. Surg. v.108 Biochemical and cellular characterization of cardiac valve tissue after cryopreservation or antibiotic preservation Lang,S.J.;Giordano,M.S.;Cardon-Cardo,C.;Summers,B.S.;Staiano-Cico,L.;Hajjar,D.P.