Ultrastructural Changes of Oocyte in Korean Catfish, Silurus asotus

발달 단계에 따른 한국산 메기(Silurus asotus) 난모세포의 미세구조적 변화

  • 윤종만 (건국대학교 동물자원연구센터) ;
  • 김계웅 (공주대학교 축산학과) ;
  • 신호철 (한국화학연구소 안전성연구센터) ;
  • 장계남 (국립수산진흥원 서해수산연구소) ;
  • 류동석 (청주대학교 생물학과) ;
  • 박홍양 (건국대학교 동물자원연구센터)
  • Published : 1995.09.01

Abstract

This study was carried out to investigate the histomorphological changes and the electrophoretic patterns of egg components, obtained from 100 of 1-year-old female catfish(Silurus asotus). Especially, the light microscopic and ultrastructural changes of ooplasm and follicular membranes of oocytes, were observed by light and transmission electron microscope. All data were collected from October in 1992 to May in 1993. The size of the nucleoli and number of the yolk granules increased as the oocyte grew. Yolk granules were deposited in the oocyte as fluid. Due to the presence of large early and late maturing oocytes, their ovaries were large, transparent, granular, and greenish in color. As the percentages of fish in LMO and RO stage increased from March to April, mean of GSI values(19.95%) increased. Follicle cells such as granulosa cell and thecal cell change a squamous into cuboid shape in LPO and EMO stage. Processes, microvilli, from the granulosa cells and from the oocyte grow and make contact with each other in the pore canals of the zona radiata during vitellogenesis, but are withdrawn as the zona radiate becomes more compact and devoid of pore canals during oocyte maturation. The electrophoretic pattern of major band in mature stage was much thicker(21k, 24k, 32k, 45k, 67∼110k, 170k dalton) than that in previtellogenic phase.

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