대한의용생체공학회:의공학회지 (Journal of Biomedical Engineering Research)

  • 제16권4호
  • /
  • Pages.387-394
  • /
  • 1995
  • /
  • 1229-0807(pISSN)
  • /
  • 2288-9396(eISSN)
대한의용생체공학회 (The Korean Society of Medical and Biological Engineering)
권호 표지

박막광도파로 센서를 이용한 산화 및 환원 혈색소의 새로운 흡광계수 측정법

A New Method for Determining the Absorption Coefficient of Oxy- and Deoxyhemoglobin by use of a Thin-fi im Optical Waveguide Sensor

  • 강신원 (경북대학교 센서기술연구소)
  • 발행 : 1995.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

박막광도파로 센서의 특소전파상수를 이용하여 인간혈액내의 산화 및 환원 혈색소의 흡광계수를 쉽고 빠르게 측정할 수 있는 방법을 제안한다. 제작된 광도파로위에 에바네센트 필드와 시료간의 상호작용길이를 변화시킬수 있도록 직렬의 다채널 시료 충전셀을 만들고 여러가지 농도의 두가지 혈색소 시료들에 대한 센서응답을 조사하여 상호작용길이에 대한 의존성을 살펴보았다. 센서응답은 상호작용길이와 시료의 농도에 선형적으로 비례한다. 시료들과 에바네센트 필드 흡수에 따른 제작된 센서의 감쇄정수를 실험적으로 구하고 제한된 방법으로 흡광계수를 결정한다. 제안된 방법으로 구한 두가지 혈색소에 대한 흡광계수는 종래의 투과광 측정법으로 구한 값과 잘 일치한다.

A simple method for determining the absorption coefficient of oxyhemoglobin and deoxyhemoglobin in human blood is proposed as an application of the complex propagation constant of a guided wave in a thin-film optical waveguide. A serial multichannel sample chamber is constructed on the waveguide to vary the interaction length between the evanescent field and the sample, and the dependence of the sensor response on the interaction length is investigated for the various concentration of two hemoglobins. The sensor response is linearly proportional to the interaction length and the concentration of two hemoglobins. The attenuation constant due to the evanescent field absorption between the samples is experimentally obtained with the designed sensor, and then the absorption coefficient is determined by the proposed method. The absorption coefficients determined by the proposed method fairly well coincided with those obtained by the conventional transmission measurement.

키워드

참고문헌

  1. IEEE Trans. BME v.31 no.11 Evaluation of Light-Emitting Diodes for Whole Blood Oximeter A.P.Shepherd;J.W.Kiel;G.L.Riedel
  2. J.Appl. Physiol. v.54 Molar absorptivities of human hemoglobin in the visible spectral range W.G.Zijlstra;Anneke Buursma;A.Zwart
  3. J. Appl. Physiol. v.52 Dependence of visible spectrum [ε (λ)] of fully oxygenated hemoglobin on concentration of hemoglobin Oswald Burkhard;Wolfgang K. R. Barnikol
  4. Clin. Chem. v.27 no.11 Determination of Hemoglobin Derivatives with the IL 282 CO-oximeter as compared with a manual spectrophotometric five-wavelength method A.Zwart;Anneke Buursma;B.Oesburg;W.G.Zijilstra
  5. Anal. Biochem. v.69 Extinction coefficients for use in equations for the spectrophotometric analysis of haemoglobin mixtures O.W.van Assendelft;W.G.Zijlstra
  6. Clin. Chem. v.20 no.2 New method for rapid determination of carboxyhemoglobin by use of double-wavelength spectrophotometry Alfred Ramieri,Jr.;Peter Jatlow;David Seligson
  7. IEEE. Trans. BME. v.27 no.3 A new instrument for the simultaneous measurement of total hemoglobin, % oxyhemoglobin, % carboxyhemoglobin, % methemoglobin, and oxygen content in whole blood Leslie J.Brown
  8. Clin. Chem. v.30 no.10 Determining methemoglobin in blood by zero-crossing-point first-derivative spectrophotometry A.Taulier;P.Levillain;A.Lemonnier
  9. Clin. Chem. v.37 no.9 Absorption spectra of human fetal and adult oxyhemoglobin, deoxyhemoglobin, carboxyhemoglobin, and methemoglobin W.G.Zijlstra;A.Buursma;W.P.Meeuwsen-van der Roest
  10. Appl. Opt. v.12 no.5 Characteristics of optical guided modes in lossy waveguides A.Reisinger
  11. J. Opt. Soc. Am. v.62 no.9 Absorption from thin-film waveguides J.N.Polky;J.H.Harris
  12. Appl. Opt. v.32 no.19 Sensitivity analysis of a thin-film optical waveguide biochenical sensor using evanescent field absorption S.W.Kang;K.Sasaki;H.Minamitani
  13. Proc. Ann. Inter. Conf. IEEE EMBS. v.13 no.4 Thin film planar optical waveguide sensor for biochemical assay S.W.Kang;K.Sasaki;H.Minamitani
  14. Appl. Opt. v.12 Measurement of thin film parameters with a prism coupler R.Ulrich;R.Torge
  15. Appl. Opt. v.19 no.17 Determining the absorption coefficient of absorbing thin films with optical waveguides K.Sasaki;H.Takahashi;Y.Kudo;N.Suzuki