Effects of PALMIWON on Cell Viability of Immune Cell and ${\beta}-cell$

취장소도세포와 면역세포에 미치는 팔미원의 영향

  • 이인순 (대구효성카톨릭대학교 약학대학) ;
  • 이인자 (대구효성카톨릭대학교 약학대학)
  • Published : 1995.10.01

Abstract

In order to investigate the usability of PALMIWON as antidiabetic immuno-modulating prescription for Insulin-dependent diabetes, we studies the effects of PALMIWON on immune cell and ${\beta}-cell$. U937 was used as the model of immune cell and RINm5F as the model of ${\beta}-cell$. The effects of PALMIWON was measured by cell viability in terms of MIT assay. As a result, PALMIWON and the compositional drugs showed the different effects m immune cell and ${\beta}-cell$. Cell viability of U937 was significantly decreased wheras that of RINm5F was no significantly difference between drug treated group and control, or significantly less reduction compared with U937. It implies that PALMIWON is useful as immunotherapeutic agents in the prevention and therapy of type 1 diabetes.

Keywords

References

  1. 한약학 대한약사한약연구회편
  2. 한방의 약리해설 박영순
  3. 동의 처방대전 1 동의 과학원
  4. 대한 면역학회지 v.12 생약 추출물의 면역 조절작용 (Ⅰ) - 도꼬마리 추출물의 면역억제작용 박승용(외)
  5. Diabetologia v.28 Molecular biology of type 1 (insulin-dependent)diabetes Lernamrk,A.
  6. Diabetologia v.32 Lessons from the NOD mouse for the pathogenesis and immunotherapy of human type 1 (insulin dependent) diabetes mellitus Lampeter,E.F.;Signore,A.;Gale,E.A.M.;Pozzilli,P.
  7. Clinical immunology and immunopathology v.59 Autoimmune diabetes in NOD mouse: suppression of immune defects by bone marrow transplantation and implication for therapy Edward,H.L.;David,V.S.
  8. Br. J. Cance. v.67 Gallium-67 radiotoxicity in human U937 lymphoma cells Jonkhoff,A.R.;Huijgens,P.C.;Versteegh,R.T.;Van Dieren, E. B.;Ossenkoppele,G.J.;Martens,H.J.M.;Teule,G.J.J.
  9. Biochem. J. v.233 Glucose-stimulated sequestration of $Ca_{2+}$ in clonal insulin-releasing cell Erik,G.;Hellman,B.
  10. Diabetologia v.35 Islet cell metabolism is reflected by the MTT (tetrazolium) colorimetric assay Janjic,D.;Wolheim,C.B.
  11. Journal of Immunological Methods v.164 Simple colorimetric cell-cell adhesion assay using MTT-stained leukemia cells Ichiro,M.;Naomi,I.;Masanari,O.;Hiroshi,K.
  12. Experimental Hematology v.21 Cellular and cytokine dependent monocytemediated leukemic cell death: modulation by interferon-γ and tumor necrosis factor-α Arjan,A.;Van de Loosdrecht;Robert,H.J.B.;Gert,J.O.;Majolein,G.B.;Mart,M.A.C.L.
  13. Experimental Hematology v.21 Apoptosis in tumor necrosis factor-α-dependent, monocyte-mediated leukemic cell death: a functional, morphologic and flow-cytometric analysis Van de Loosdercht, A. A.;Ossenkoppele,G.J.;Beelen,R.H.J.;Broekhoven,M.G.;Drager,A.M.;Langenhuijsen,M.M.A.C.
  14. Diabetes v.37 Interferon-γ-Induces class Ⅱ MHC antigens on RI$N_{m}$5F cells Anne-Marie Varey;Peter M. Lydyard;Betty M. Dean; Peter H. van der Meide;Jouce D. Baird;Anne Cooke
  15. Diabetes v.37 Evidence for initial involvement of macrophages in development of insulitis in NOD mice Lee,K.U.;Amano,K.;Yoon,J.W.
  16. Diabetes v.37 Preferential infiltration of macrophages during early stages of insulitis in diabetes-prone BB rats Lee,K.U.;Kim,M.K.;Amano,K.;Pak,C.Y.;Jaworski,M.A.;Mehta,J.G.;Yoon,J.W.
  17. Diabetes v.39 Studies on autoimmunity for initiation of β-cell destruction Amano,R.;Yoon,J.W.
  18. Diabetologia v.32 Macrophage infiltration precedes and is a prerequisite for lymphocytic insulitis in pancreatic islets of pre-diabetic BB rats Haneberg,H.;Kolb-Bachofen. V.;Kantwerk-Funke,G.;Kolb,H.
  19. Diabetologia v.30 Prevention of lymphocytic thyroiditis and insulitis in diabetes-prone BB rats by the depletion of macrophages Lee,K.U.;Pak,C.Y.;Amano,K.;Yoon,J.W.
  20. Experimental Hematology v.21 Spectrophotometric determination of clonogenic capacity of leukemic cells in a semisolid microtiter culture system Schweitzer,C.M.;Van de Loosdrecht, A. A.;Jonkhoff,A.R.;Ossenkoppele,G.J.;Huijgens,P.C.;Drager,A.M.;Broekhoven,M.G.;Langenhuijsen,M.M.A.C.
  21. Diabetologia v.35 Islet cell metabolism is reflected by the MTT (tetrazolium) colorimetric assay Janjic,D.;Wollheim,C.B.
  22. Pharmacology & Toxicology v.61 The Partial protective effect of branched chain amino acids against streptozotocin-Induced cytotoxicity to mouse pancreatic islets in vitro Decio,L.E.;Stellan,S.