YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Assignment of the Carbonyl Carbon Resonances in Anti-Dansyl Antibodies

항 단실 항체의 카르보닐탄소 유래 시그날의 귀속

  • ;
  • ;
  • Koichi Kato (Faculty of Pharamaceutical Sciences, University of Tokyo) ;
  • Yoji Arata (Faculty of Pharamaceutical Sciences, University of Tokyo)
  • Published : 1995.10.01
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Abstract

The anti-dansyl antibodies were specifically labeled with stable isotope by growing hybridoma cells in serum-free medium. Assignments of the observed carbonyl carbon resonances have been determined by using $^{13}C-{15}N$ double labeling method in order to assign the Leu resonances. However, when the identical dipeptide appears more than twice in the polypeptide sequences, we applied the proteolytic fragments in the fragment-specific method. Carboxypep-tidase B-treated antibody has also been used to assign the Lys-447 in C terminal amino acid. These unambiguously assigned carbonyl carbon resonances in antibodies are thought to be useful in elucidating not only the structure of antibodies but also the structure-function relationship in the antibody by $^{13}C$ neuclear magnetic resonance spectroscopy.

Keywords

References

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