YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
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Optimization for the Solid-Phase Extraction of Trace Clobazam from Biological Samples

생체시료로부터 미량 크로바잠의 고상추출을 위한 최적화

  • Kim, Kyoung-Rae (College of Pharmacy, Sung Kyun Kwan University) ;
  • Kim, Jeong-Ho (College of Pharmacy, Sung Kyun Kwan University) ;
  • Kim, Hyub (College of Pharmacy, Sung Kyun Kwan University) ;
  • Yoon, Hye-Ran (College of Pharmacy, Sung Kyun Kwan University)
  • 김경례 (성균관대학교 약학대학) ;
  • 김정호 (성균관대학교 약학대학) ;
  • 김협 (성균관대학교 약학대학) ;
  • 윤혜란 (성균관대학교 약학대학)
  • Published : 1995.08.01
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Abstract

The sorption and desorption properties of four adsorbents were evaluated for the trace enrichment of clobazam from biological samples. Graphitized carbon black(GCB) gaved the highest dynamic adsorption coefficient. Among the six organic solvents examined, acetone gave the highest desorption coefficient for the clobazam adsorbed on GCB. Using the GCB column, the optimum elution volume of the eluting solvent was evaluated from the on-line monitored breakthrough curve for clobazam. When GCB as the solid adsorbent and acetone as the eluting solvent were used for the solidphase extraction of clobazam from serum, the recoveries were higher than 83% with good reproducibility in the concentration range of 20-50 $\mu\textrm{g}$/ml.

Keywords

References

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