마늘의 조직 센서를 이용한 L-asparagine의 정량

Determination of L-asparagine Using a Garlic Tissue Electrode

  • 발행 : 1995.04.01

초록

Garlic tissue cells are employed for the conversion of L-asparagine into ammonia. An ammonia gas electrode is used as a detector. The effect of pH, buffer solution, temperature and life time of electrode to have used were investigated in order to optimize the electrode response. The combination of L-asparaginase in garlic tissue cells and the gas electrode response linearly to Lasparagine over the concentration range 1.0$\times$10$^{-4}$~1.0$\times$10$^{-1}$ M with a slope of 72.0 mV/decade and is selective with respect to other L-amino acids.

키워드

참고문헌

  1. Ann. NY Acad. Sci. v.102 Electrode systems for continuous monitoring in cardiovascular surgery Clark,L.C,Jr.;Lyons,C.
  2. Anal. Chem. v.56 Ferrocene-mediated enzyme electrode for amperometric determination of glucose Cass,A.E.G.;Davis,G.;Francis,G.D.;Hill,H.A.O.;Aston,W.J.;Higgins,I.J.;Plotkin,E.V.;Scott,L.D.L.;Tuner,A.P.F.
  3. Anal. Chem. v.54 Galactose oxidase enzyme electrode with internal solutions potential control Johnson,J.M.;Halsall,H.B.;Heineman,W.R.
  4. Am. Biotechnol. Lab. v.13 A multipurpose enzyme sensor based on alcohol oxidase Hopkin,T.R.
  5. Trends in Anal. Chem. v.3 Microbial sensor for screening mutagens Karube,I.
  6. Biotechno. Bioeng. v.25 NO₂sensor which use immobilized nitrite oxidizing bacteria Okada,T.;Karube,I.;Suzuki,S.
  7. Anal. Chem. v.54 Optimization of a tissue-based membrane electrode for guanine Arnold,M.A.
  8. Anal. Chem. v.53 Selectivity enhancement of a tissue-based adenosine sensing membrane electrode Arnold,M.A.
  9. J. Kor. Chem. Soc. v.38 The biosensors for L-glutamine using tissue slices of Wistar rat Bae,J.H.;Choi,S.H.;Lim,D.J.;Kim,U.R.
  10. Anal. Chem. v.54 Heterogenous immunoassay for serum proteins by differential pulse anodic stripping voltammetry Dolyle.M.J.;Halsall,H.B.;heinman,W.R.
  11. Anal. Chem. v.56 Potentiometric digoxin antibody measurements with antigen ionophores based membrane electrode Keating,M.Y.;Rechnitz,G.A.
  12. Anal.Chem. v.8 Neuronal Biosensors (review) Buch,R.M.;Rechnitz,G.A.
  13. Archives of Microbiology v.31 Different forms of quinoprotein aldolase-(glucose) dehydrogenase Duine,J.A.;Frank,J.;Van der Meer,R.
  14. J. Chromatogr. v.455 Liquid chromatographic determination of alliin in garlic and garlic products Emiko,M.;Akira,N.;Yoshimi,K.;Koichi,S.