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The Pharmaceutical Society of Korea (대한약학회)
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Effects of Ginsenosides on the Glutamate Release and Intracellular Calcium Levels in Cultured Rat Cerabeller Neuronal Cells

  • Oh, Seikwan (Department of Pharmacology and Toxicology) ;
  • Kim, Hack-Seang (Department of Pharmacology, College of Pharmacy, Chungbuk National University) ;
  • Seong, Yeon-Hee (College of Veterinary Medicine, Chungbuk National University)
  • Published : 1995.10.01
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Abstract

These studies were designed to examine the effects of ginsenosides on glutamate neurotansmission. In primary cultures of rat cerebellar granule cells, ginsenosides (Rb1, Rc did not Rg1, $500\mug/ml$) increased glutamate release which was measured by HPLC. but HPLC, but Re did not shwo an elevation of glutamate release. However, all of these ginsenosides down-regulated N-methyl-D-aspartate (NMDA)-induced glutamate release. Rc strongly increased glutamate release and elevated intracellular clcium concentrations $([Ca_{2+}]_i)$ which was measured by ratio fluorometry with FURA-2AM. These results indicate that ginsenosides have a homeostatic effect on glutamate neurotransmission, and there is a structure-function relationship among the ginsenosides tested.

Keywords

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