Parasites, Hosts and Diseases
- Volume 33 Issue 4
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- Pages.387-390
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- 1995
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
Axenization of Entamoeba histolytica, a Korean strain YS-27
이질아메바 한국분리주 YS-27의 무균 배양화
- Jang, Jae-Gyeong (Institute of Tropical Medicine & Department of Parasitology, Yonsei University College of Medicine) ;
- Im, Gyeong-Il (Institute of Tropical Medicine & Department of Parasitology, Yonsei University College of Medicine) ;
- So, Jin-Tak (Institute of Tropical Medicine & Department of Parasitology, Yonsei University College of Medicine)
- 장재경 (연세대학교 의과대학 열대의학연구소 및 기생충학교실) ;
- 임경일 (연세대학교 의과대학 열대의학연구소 및 기생충학교실) ;
- 소진탁 (연세대학교 의과대학 열대의학연구소 및 기생충학교실)
- Published : 1995.12.01
Abstract
In the present communication, YS-27, a Korean strain of Entnnloeba histoIWtica is described for the isolation and establishment of axonic cultivation. 5. histoLvticc, designated as strain "YS-27" was isolated from the pus of a hepatic abscess obtained from a 72 you old inpatient of August 10, 1969. Specimens, were obtained by needle aspiration, inoculated immediately and weekly cultured in a modified diphasic medium at 37℃. Strain YS-27 had been maintained for more than 15 years by weekly subculture until February, 1985. These cultures were transferred to a monoxenic TTY-SB medium seeded with a trypanosomatid of the genus CyithidiG. Penicillin G, 2 to 10 H 103 International units and Streptomycin, 2 to 10 mg per 100 ml, were added to the cultures to eliminate the bacteria. After more than one year later, these two organisms were well maintained by transfer every 3 or 4 days until .January. 1986 at 37℃ in TTY-SB medium in the absence of other microorganisms. These monoxenic cultures were then transferred to TYI-S-33 medium. Strain YS-27 alone had not been growing at the time of transfer, but when overlaid with Crithinia at intervals of 3 to 4 days, strain YS-27 propagated well. The Clthidio died out several weeks later after several passages. Beginning in April, 1986, strain YS-27. was successfully established in axonic culture in TYI-S-33 medium and has been maintained in continuous culture and multiplied well to present.
1969년 8월 10일 서울 고려병원의 입원친자 김00의 간농양에서 채취한 고름을 modified diphasic배지(Faust and Russell 1964)에서 배양하여 이필아메바 한국븐리쿠 YS-27을 얻었다. 이를 1985년 2월부터 TTY-53 배지에 넣어 Crithidia와 함께