Ultrastructural antigenic localization in Paragonimus iloktsuenesis during developmental stage by immunogold labeling method

면역황금표지법에 의한 일록춘폐흡충의 발육단계별 항원성부위

Antigenic localization in Parofonimn iloktsuenensis worm tissues (tegument, intestine and vitelline gland) in different developmental stages of 2 weeks, 3 weeks, 4 weeks, 5 weeks and 33 weeks from albino rats (Sprague-Dawley) infected with P iloktsuenensis was observed by electron microscopy. These worm tissues of different developmental stage of R iloktsuenensis was observed on electromicrograph by immunogold labeling method using R iLoktsuenensis infected rat serum of 10 weeks. Antigenic localization was demonstrated as labeling of gold particles in tissues on electronmicrograph. In tegument, gold particles were labeled on tegumental tissue, generally more numerous on secretory granules in tegumental syncytium 2 weeks than those on the other elder developmental stages, but there was a little variation in antigenicity according to individual worm tissue. In general, antigenicity in tegumental tissue was not strong (gold particles: 0.1-5/1 Mm2). In intestine, a large number of gold particles (15-18/1 Mm2) were labeled in intestinal epithelium. Gold particles were concentrated especially on secretory granules in cytoplasm, and gold particles were labeled not only in cytoplasmic protrusions, but also in intestinal luminal contents. Intencity of labeling of gold particles was not correlated with developmental stage of worms. In vitelline gland, a large number of gold particles were labeled on vitelline globules. The gold particles in vitelline globules (8- 11/1 Mm2) were concentrated in protoplasm among segmental globules . Key words: Pnragonimus iloktsuenensis, immunogold labeling method, tissue antigen ultrastructure.

일록춘폐흡충(Poragonimus iloktsuenensis)의 표피층, 장상피층 및 난황선의 조직항원 성을 발육단계별로, 면역황금표지법(immunogoldlabelingmethod)으로 관찰하였다. 일록춘폐흡충 으로 감염건 횐쥐(Sprague-Dawley)로 부터, 감염후 2주 3주, 4주, 5주 및 33주에 적출한 충체를 항원으로 사용하였고, 감염 후 10주의 흰쥐혈청을 항체로 사용하였다 조직항원성은 조직에 표지된 황금입자로 판정하였다. 표피층은 유약충으로부터 성충에 이르는 발육과정중 전반적으로 황금입자 표지가 많지 않았고 충체가 성숙되어감에 따라서 황금입자 표지가 다소 드물어지는 경향이 있었으나, 충체 개체별 항원 강도에 차이가 많았다. 장상피층과 난황선은 충체의 발육단계에 관계없이 많은 수의 황금입자가 표지되어 강한 항원성을 나타내었다. 이 실험의 결과, 일록춘폐흡충의 표피층 장강피층, 및 난황선은 중요한 조직항원 부위로 보이나, 표피층은 항원성이 낮은 편이고, 발육단계 및 개체별로 항인강도에 차이가 있었다. 장상피층 및 난황선도 개체별로 황금입자 표지강포에 다소의 차이는 있었으나 충체의 발육단계에 관계없이 계속해서 강한 항원성이 유지되는 조직부위로 보인다.