Isolation of Bacillus sp. Producing ${\beta}-Galactosidase$ with High Transgalactosylation Activity and its Culture Characteristics Regarding Enzyme Production

갈락토스 전이활성이 높은 ${\beta}-galactosidase$ 생산균의 분리 및 효소생산과 관련된 몇가지 특징

A Bacillus strain which produces ${\beta}-galactosidase$ with high transgalactosylation activity, was isolated from soil and tentatively designated as Bacillus sp. A1. When ${\beta}-galactosidase$ from Bacillus sp. A1 reacted with 40% (w/w) lactose, transgalactosylation ratio reached up to 90% at the 70% conversion of the initial lactose. The biosynthesis of the enzyme in Bacillus sp. A1 required lactose as an inducer and was repressed by glucose. Observing that the addition of amino acids to culture medium resulted in enhancing, to a significant extent, both the growth and the enzyme production of the strain, yeast extract and commercially available hydrolysates of protein were examined for the suitability as amino acid source. As it turned out, SMP, an enzymatic hydrolysis product of soybean protein from Fuji Oil Co.(Japan), was the most suitable for optimization of the culture medium. When Bacillus sp. A1 was cultured in the presence of 0.5% SMP and 2% lactose, the enzyme activity increased up to $1.8\;U/m{\ell}-broth$.

토양으로부터 갈락토스 전이활성이 우수한 ${\beta}-galactosidase$를 생산하는 미생물을 분리하고 부분동정하여 Bacillus sp. A1으로 명명하였다. 선발된 균주가 생산하는 효소는 40%(W/W) 유당용액에서 초기유당의 70%가 전환되었을 때 90%의 높은 전이율을 보였다. 효소의 생합성은 유당에 의하여 유도되었고 포도당에 의하여 저해받았다. 효소생산을 위한 탄소원으로는 유당이, 유기질소원으로는 효소분해 대두단백질이 적합하였다. 탄소원과 유기질소원을 최적화하므로써 배양된 균주의 효소활성은 최소배지에서 배양된 효소활성과 비교하여 약 3배 이상($0.5\;U/m{\ell}-broth$에서 $1.8\;U/m{\ell}-broth$로) 증가하였다.