Constituents of Antimutagenic Factor from Brown Rice

현미의 항돌연변이 활성물질의 구성성분

To investigate the constituents of antimutagenic factor from brown rice, methanol extracts were fractionated into ether, ethylacetate, buthanol and aqueous fractions. The ether fractions showed distinct antimutagenic effect and active spot were selected by silica gel chromatography. The specific activity of active spot decreased with isolation of the active components from the methanol extract. Qualitative analyses of the active spot by using various spraying reagents revealed that ninhydrin and orcinol did not develop colored reactions. But, ferric chloride, 2,7-dichlorofluorescein, antimony pentachloride, phosphomolybdic acid, bromothymol blue and rhodamine 6G led to colored reactions. These results suggested that the consitituents of active material were neither polar nor nitrogen-containing compounds and that they may contain phenolic compounds and fatty acid derivatives. Main compounds of the active spot were analyzed to be o-hydroxy benzyl alcohol(saligenin), octanoic acid(caprylic acid), 9,12-cis-octadecadienoic acid(linoleic acid), 11-cis-octadecenoic acid(oleic acid), hexadecanoic acid(palmitic acid), 1H-indole-2-carboxylic acid and 1,2-benzenedicarboxylic acid(phthalate) in GC/Mass spectrum, and antimutagenicity of these active compounds using standard regeant was reconfirmed in S. typhimurium reversion assay.

한국산 쌀(일반계 일품벼)로부터 돌연변이 억제물질의 구성성분을 확인할 목적으로 methanol 추출물을 용매분획 및 column chromatography하여 활성 spot을 분리하고 정성 분석과 기기분석을 수행하였다. Ether, ethylacetate, butanol 및 수용성 용매 획분 가운데 ether 획분의 억제활성이 가장 높았으며 silica gel column chromatography를 시행하여 성분을 분리하였을 경우 methanol추출물의 억제활성이 80% 이상으로 나타났던 결과와 비교하여 분리가 진행됨에 따라 억제활성은 $0{\sim}30%$로 감소하였다. 활성 물질을 정성분석한 결과 ferric chloride, 2,7-dichlorofluorescein, antimony pentachloride, phosphomolybdic acid, bromothymol blue, rhodamin 6G등의 시약에서는 양성을, ninhydrin, Bial's orcinol 시약에서는 음성을 나타내어 억제활성 물질은 페놀성 화합물과 지질성분인 것으로 조사되었다. 분리된 활성획분을 GC/Mass로 확인한 결과 o-hydroxy benzyl alcohol (saligenin), octanoic acid(caprylic acid), 9,12-cis-octadecadienoic acid(linoleic acid), 11-cis-octadecenoic acid(oleic acid), hexadecanoic acid(palmitic acid), 1H-indole-2-carboxylic acid, 및 1,2-benzenedicarxylic acid(phthalate)가 주요 구성성분인 것으로 분석되었으며 이들 성분들의 억제활성은 표준시약을 사용한 항변이원성 시험에서 재확인되었다.