Applied Biological Chemistry
- 제38권5호
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- Pages.387-392
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- 1995
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
대두 원형질체와 형질전환된 담배에서의 대두 glycinin 유전자 Gy2 promoter의 발현조절 기작
Analysis of the Glycinin Gy2 Promoter Activity in Soybean Protoplasts and Transgenic Tobacco Plants
- Kim, Soo-Jung (Department of Agricultural Chemistry, Seoul National University) ;
- Lee, Jee-Young (Department of Agricultural Chemistry, Seoul National University) ;
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Kim, Chung-Ho
(Department of Food and Nutrition, Seowon University) ;
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Choi, Yang-Do
(Department of Agricultural Chemistry, Seoul National University)
- 발행 : 1995.10.31
초록
대두 glycinin 유전자의 조직 특이적이고 분화 발달 특이적인 발현 조절 메카니즘을 연구하기 위하여 Gy2 유전자의 5' upstream 부위 염기서열을 조사한 결과, glycinin 유전자의 발현을 조절하는 인자로 여겨지는 여러 가지 조절 인자들을 발견하였다. 진핵세포 유전자에 공통적으로 존재하는 TATA box와 AGGA box가 존재하고, 종자 저장 단백질에서 공통적으로 발견되는 embryo factor binding sequence, RY repeat CACA sequence,
To study the regulatory expression mechanism of soybean glycinin gone, Gy2, the 5' upstream region of the gene was searched for the presence of putative regulatory elements by nucleotide sequencing. It revealed various kinds of regulatory sequence elements commonly found in plant storage protein genes. There were canonical promoter sequences, TATA box (TATAAT) and AGGA box (GAAT) which are common in the 5' upstream region of the plant genes. The embryo factor binding sequence, RY repeat, CACA sequences,