Isolation of Cytotoxic Substances from Chysanthemum Boreale M.

산국으로부터 항암활성 성분의 분리

  • Yang, Min-Suk (Department of Agricultural Chemistry, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Nam, Sang-Hae (Department of Agricultural Chemistry, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
  • 양민석 (경상대학교 농화학과, 식물분자생물학연구소) ;
  • 남상해 (경상대학교 농화학과, 식물분자생물학연구소)
  • Published : 1995.06.30

Abstract

Sixty kinds of medicinal plants were examined upon the cytotoxicity against L1210 mouse leukemia cells. Isolation and purification of effective antitumor substances from Chysanthemum boreale had been performed which appeared strong cytotoxicity, In cytotoxicity test of the each solvent fractions, $ED_{50}$ values of chloroform fraction against L1210, K562 and A549 cells were shown as 3.98, 4.28, 3.84 (${\mu}g/ml$), respectively. Compound I and II were purified from the chloroform fraction. Between the purified compounds, $ED_{50}$ values of Compound I against L1210, K562 and A549 cells were shown as 0.55, 0.0003, 0.001 (${\mu}g/ml$), respectivety. Whereas Compound II was shu as 4.79 against K562.

60종의 생약의 methanol 추출물을 L1210세포에 대하여 1차스크리닝실험을 수행하여 항암활성물질을 검색하고, 그 중에서 비교적 세포독성이 강하게 나타나는 산국(Chrysanthemum boreale M.)에서 항암활성물질을 분리정제하였다. 산국의 용매분획물의 L1210, K562, A549세포에 대한 세포독성실험에서는 chloroform 분획에서 $ED_{50}$값이 각각 3.98, 4.28, 3.84 (${\mu}g/ml$)로 나타났다. 이 chloroform 분획에서 유효세포독성물질을 정제하여 Compound I과 Compound II를 각각 얻었으며, 이 중에서 Compound I은 L1210, K562, A549세포에 대하여 각각 $ED_{50}$값이 0.55, 0.0003, 0.001 (${\mu}g/ml$)로 강한 세포독성을 나타내었으나, Compound II는 K562에 대해서만 $ED_{50}$ 값이 4.79 (${\mu}g/ml$)의 세포독성을 나타내었다.

Keywords