Comparison of the Two in Vitro Cytotoxicity Assays in Primary Cultured Rat Hepatocytes - the Neutral Red (NR) and Lactate Dehydrogenase (LDH) Tests

흰쥐의 배양 간세포를 이용한 세포독성시험에 있어서 뉴트랄레드 및 젖산 탈수소효소법의 비교

The acute cytotoxicities of chloroquine sulfate, propranolol, ascorbic acid, acetylsalicylic acid and acrylamide on cultured adult rat hepatocytes were evaluated by the use of LDH leakage and NR uptake test. On the basis of $IC_{50}$ values, the rank order of cytotoxicities of these drugs in both tests was chloroquine sulfate > propranolol > acetylsalicylic acid > ascorbic acid. The $IC_{50}$ of LDH test was very similar to that of NR uptake test. Thus, we concluded that both tests are reliable and sensitive methods in detecting toxicity in adult cultured rat hepatocytes.